Calibrate the Pipettes
Once your labware is calibrated and saved on your deck, you'll need to calibrate your pipettes so your robot can accurately deliver the volumes you need! This is all done in the Pipette tab of the app.
When calibrating pipettes, you'll save four positions: Top, Bottom, Blowout and Drop Tip. Make sure you have a tip on your pipette before beginning this process! Also, make sure you have your pipette plunger set to deliver the full volume range for which it is rated (ie, for the p200, twist the plunger to set the dial at 200 microliters).
The Bottom position is the same as the "soft" stop on your pipette. You should jog the actuator down until the plunger is depressed to that position, then use the green "save" button next to "Bottom" to save this position.
**Pro Tip - use a pen to mark the soft stop position on your pipette, then you can just jog down until the markings line up!**
Blowout should be set 1-2 mm below the Bottom position. This is the little extra push to get that last bubble of liquid out of your pipette tip.
Once you've saved the rest of your positions, keep jogging the actuator down in small increments until the drop tip screw fully depresses the button on your pipette and the tip is ejected. Make sure to save this position as well before moving on to testing.
Once these positions are all saved, you can test your calibrations. This is most easily done gravimetrically using a small scale. Use the test button to pick up liquid and eject it onto the scale. If this volume isn't exactly what you expect, maybe 203 uL instead of 200, no big deal! You can go into the Pipette tab and input the value the scale gives you. The robot will adjust its calculations accordingly and will then be able to dispense the correct volumes during experiments!