A colorimetric assay used to determine the concentration of proteins in a solution. The primary purpose of the BCA Assay is to quantify the amount of protein present in a sample, which is essential for various downstream applications in molecular biology and proteomics.
Workflows where BCA Assay is needed
Protein purification processes to determine the yield and purity.
Before gel electrophoresis to ensure equal protein loading.
Prior to protein labeling or modification to ensure the correct amount of protein is used.
In cell culture studies to determine protein expression levels.
Manual pipetting slows scientific discovery
These are machines designed to automatically pipette liquids into designated containers, ensuring accuracy and consistency.
Some platforms integrate the entire process, from sample addition to reading the absorbance, streamlining the workflow.
Automation software can be used to set up protocols, ensuring that the assay is performed the same way every time.
The OT-2 is a bench-top liquid handler designed to be accessible and flexible enough to automate many common applications.
The Opentrons Flex Absorbance Plate Reader Module is a fully automated solution for absorbance-based assays. Using the example of protein quantification by BCA assay, our scientists will show how to use an Opentrons Flex with the newly released on-deck absorbance plate reader to set up and run assays, and then normalize concentrations, all from a single protocol without human intervention.
Our team of experts can help figure out if automation is right for you. Book a virtual demo to discuss your workflow needs with an expert.
