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Get the Opentrons App

Download for use with OT-2 and Opentrons Flex.

  • Opentrons Flex or OT-2 Robot
  • Connect the app to your robot via USB 2.0 or Wi-Fi

Operating System

  • Windows 10 or later
  • macOS 10.10 or later
  • Ubuntu 20.04 or later
Installation Instructions

Visit this support article for detailed installation instructions, or check our operating system requirements.

Robot Operating System Update

Robot Operating System updates require the computer running the Opentrons App to connect to the Internet. If that computer does not have access to the internet, you can download the latest Robot Operating System here.


View this support article for detailed manual robot software installation instructions.

OT-One App

Download for use with OT-One robot

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  • Opentrons OT-One Robot
  • USB 2.0
Operating System

  • Windows 10 or later
  • macOS 10.9 or later
  • Ubuntu 12.04 or later
API Documentation

The Opentrons API is a simple framework designed to make writing automated biology lab protocols easy. Check out the documentation and start writing your own protocols.

Protocol Library

A community generated repository of commonly used protocols, including PCR, transformations, dilutions, and gene editing. Download a protocol to run in the OT App.