Opentrons logo
Cancel
Tips & Labware Services Go to Shop
NGS Nucleic Acid Extraction PCR Protein Purification ELISA View All
OEM Partnerships
Knowledge Hub Customer Stories Citations Getting Started FAQs
Mission Events & Webinars News Careers Contact Us
Protocol Designer Protocol Library Python API Labware Library GitHub OT App
Schedule a Demo Contact Sales
The next generation of lab automation is here! Discover all the ways the new Opentrons Flex fits your lab

Automating NGS Library Prep

NGS is a powerful tool, but many parts of the NGS workflow are complex, require a lot of repetitive pipetting and are prone to errors. Labs are increasingly turning to automation to help with their NGS workflows.

Watch Video
ngs

Thinking through the transition to NGS automation

Every lab has its own resources and goals. When evaluating automation, you need to think through the transition in the context of your lab and your samples. This guide will help you decide if automating NGS is the right decision for you.

DOWNLOAD THE EBOOK

NGS Workstation

Automate your NGS workflow on the OT-2

Learn More
ngs workstation

The OT-2 can automate your library prep and pre-sequencing workflows using any leading reagent or magnetic bead-based system on the market. Explore our popular NGS workstation, which includes all the hardware modules and consumables you need to start automating NGS immediately.

09274
Webinar & Application Note

Library Prep on the OT-2

Fully Automated NGS Library Preparation with the OT-2

Watch Webinar Download App Note
37842351
Webinar & Application Note

Quantification, Normalization, and Pooling on the OT-2

Fully Automated NGS

Watch the Webinar Download App Note
“

We automated much of the [OCTOPUS plasmid sequencing] protocol using the open-source Opentrons OT-2 liquid handling system and reduced the necessary hands-on time by more than 70%.

– Bryan Jiang, Octant

OCTANT

Research Spotlights

NGS

Microbial WGS on the OT-2

Genome Biology

A low-cost, high-throughput bacterial isolation and genome sequencing method for characterizing genomic diversity within gut microbiota

Read Paper
Liquid Handling

Choosing a liquid handler

BMC Genomics

A review article outlining considerations for genomics labs looking to automate their NGS workflows

Read Paper
NGS

Point-n-Seq library prep

Application Note

Data demonstrating automation of Avida Biomed’s hybridization-based targeted sequencing technology on the OT-2

Read App Note

Resources

Reagent Application Data Protocols Verification *
2S Turbo DNA Library Kit (rebranded as xGEN)
Rapid High Quality Next Generation Sequencing Library Preparation With Swift 2S Turbo DNA Library Kits On The OT-2
Swift 2S Turbo DNA Library Kit Protocol: Fully Automated
Swift 2S Turbo DNA Library Kit Protocol: Semi Automated
Opentrons
Partner
Omega Bio-tek Mag-Bind TotalPure NGS
Fully automated NGS clean up and size selection with Omega Bio-Tek Mag-Bind TotalPure NGS on the OT-2
NGS Cleanup and Size Selection with Omega Bio-tek Mag-Bind TotalPure NGS
Opentrons
Partner
Illumina DNA Prep Kit
Automated library preparation of small genomes using DNA preparation kits
Opentrons
Roche KAPA HyperPrep Kit
Automated library preparation of small genomes using DNA preparation kits
Roche HyperPrep
Opentrons
NEBNext Ultra II DNA Library Prep Kit for Illumina
Automated library preparation of small genomes using DNA preparation kits
NEBNext Ultra II DNA Library Prep Kit for Illumina
Opentrons
Partner
NEBNext Ultra II FS DNA Library Prep Kit for Illumina
NEBNext Ultra II FS DNA Library Prep Kit for Illumina (4-part protocol)
Community
NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (4-part protocol)
Community
xGen DNA Library Prep MC & EZ Kits
IDT xGen MC protocol
IDT xGen EZ protocol
Opentrons
Twist Library Preparation Kit
Twist library prep (3-part protocol)
Community
Nextera XT DNA Library Preparation Kit
Illumina Nextera XT (4-part protocol)
Community
AMPure XP
NGS Library Cleanup with AMPure XP Beads
Community
Illumina DNA Prep with Enrichment Kit
Illumina DNA Prep with Enrichment (2-part protocol)
Community
Zymo-Seq RiboFree Total RNA Library Prep
Zymo RiboFree Total RNA Library Prep (4-part, 2-robot protocol)
Community
EZ DNA Methylation Gold Kits
EZ DNA Methylation Gold (5-part protocol)
Community
Oxford Nanopore Rapid Barcoding Kit
Oxford Nanopore Ligation Sequencing Kit (2-part protocol)
Community
seqWell plexWell LP 384
plexWell LP 384 (4-part protocol)
Community
Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina
Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina protocol
Community
Ion Torrent Oncomine Focus Assay
Oncomine Focus Assay (4-part protocol)
Community
Applied Biosystems AgriSeq HTS Library Kit
AgriSeq HTS Library Kit (4-part protocol)
Community
Swift Rapid RNA Library Kit (rebranded as xGEN)
Swift Rapid RNA Library Kit (5-part protocol)
Community
QIAGEN QIAseq Targeted RNAscan Panels
QIAseq Targeted RNAscan Panels protocol
Community

*Verification categories:
Opentrons = has been verified by the Opentrons Team, who verify that the scientific results are as expected.
Partner = has been verified by our reagent partner, who verifies that the scientific results are as expected.
Community = protocol is in use by members of the Opentrons community.

Demo

Want to know more?

Our team of experts can help figure out if automation is right for you. Book a virtual demo to discuss your workflow needs with an expert.

Schedule Demo