A technique where DNA is fragmented and tagged with adapter sequences simultaneously, primarily for efficient DNA library preparation for next-generation sequencing (NGS). The process is crucial for workflows such as DNA sequencing, targeted studies, metagenomics, and chromatin profiling like ATAC-seq.
The primary purpose of DNA Tagmentation is to prepare DNA libraries for next-generation sequencing in a rapid and efficient manner. The process streamlines library preparation, reduces the time and steps required, and can result in more uniform coverage during sequencing. DNA Tagmentation is commonly used in various workflows such as:
The OT-2 is a bench-top liquid handler designed to be accessible and flexible enough to automate many common applications.
Opentrons helps you automate DNA Tagmentation with open-source protocols for the OT-2 and Opentrons Flex