Enzyme-linked immunosorbent assays (ELISAs) are widely used analytic tools for detection of a target molecule in liquid samples. Most assays include multiple pipetting steps and incubations that require a high level of consistency in sample handling. Automation can help you complete these intensive assays with reduced hands-on time while maintaining consistent results.
The Opentrons Flex Absorbance Plate Reader Module is a fully automated solution for absorbance-based assays. Using the example of protein quantification by BCA assay, our scientists will show how to use an Opentrons Flex with the newly released on-deck absorbance plate reader to set up and run assays, and then normalize concentrations, all from a single protocol without human intervention.
Get an overview of automation methods and see data from automating a range of different ELISA assays, including a sandwich EIA for detection of human fibronectin (FN), two competitive ELISAs and a bead-based ELISA for quantifying SARS-CoV-2 antibodies.
Automate competitive and sandwich ELISAs for various target molecules for up to 96 samples on the OT-2
Automate liquid transfers, washing and shaking steps for up to 96 samples on the OT-2
Our team of experts can help figure out if automation is right for you. Book a virtual demo to discuss your workflow needs with an expert.
