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TUNEL Assay

TUNEL (Terminal deoxynucleotidyl Transferase dUTP Nick End Labeling) Assay is a method used in molecular biology and proteomics to detect DNA fragmentation resulting from apoptotic signaling cascades. This assay is particularly important for understanding the mechanisms of cell death and for identifying and quantifying apoptotic cells within a given cell population. It works by labeling the ends of fragmented DNA, which can then be visualized by various methods, indicating cells undergoing apoptosis.

Workflows Requiring the TUNEL Assay:

 

How to Perform a TUNEL Assay:

 

Step 1. Sample Preparation

 

Step 2. TUNEL Reaction

 

Step 3. Detection

 

Step 4. Imaging and Analysis

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How to Automate the TUNEL Assay Process

Automating the TUNEL Assay can involve the use of liquid handling robots and automated microscopy for imaging. 

 

Advantages of TUNEL Assay

 

Additional Apoptosis Detection Methods

Apart from the TUNEL Assay, various assays provide a comprehensive understanding of apoptosis by focusing on distinct aspects or stages of the cell death process: