Proteomics throughput is increasing, driven by advances in resolution, cost, and speed. Manual pipetting during sample preparation not only wastes time, but has become a bottleneck limiting research, leading labs all over the world to embrace automation.
The Opentrons Flex Absorbance Plate Reader Module is a fully automated solution for absorbance-based assays. Using the example of protein quantification by BCA assay, our scientists will show how to use an Opentrons Flex with the newly released on-deck absorbance plate reader to set up and run assays, and then normalize concentrations, all from a single protocol without human intervention.
Save time by downloading free protocols from the Opentrons Protocol Library. Select from a range of protein extraction (including SP3), quantification, and proteomic sample preparation methods including digestion and normalization.
Automation of three ELISA protocols on the Opentrons OT-2 with an on-deck Heater-Shaker Module
Automation of bead-based ELISA forSARS-CoV-2 neutralizing antibody on the Opentrons OT-2 with an on-deck Heater-Shaker Module
Automated Protein Purification with Biotage Dual Flow Chromatography PhyTip Columns on the OT-2
Automated Ni-NTA bead-based protein purification on the OT-2 liquid handler
A Semiautomated Paramagnetic Bead-Based Platform For Isobaric Tag Sample Preparation