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OT-2 PCR Workstation

From $20,300

PCR setup and thermocycling for up to 96 samples

The PCR Workstation includes OT-2 Robot, Pipettes, Tips, Labware, Thermocycler (optional), Temperature Module and Custom Protocol Development

With the OT-2 PCR Workstation, you can automate your end-point PCR setup and thermocycling workflows for up to 96 samples. Configure your workstation with an on-deck Thermocycler for end-to-end automation, or without a Thermocycler for automated PCR setup with off-deck thermocycling.


PCR Setup



What’s in the box

The OT-2 PCR Workstation includes all the hardware and consumables you need to perform automated end-point PCR. It comes with Opentrons’ protocol development service, which provides you with a customized protocol unique to your PCR assay. You’ll also enjoy free access to the Opentrons Protocol Designer, Python API, and Protocol Library.



  • OT-2
  • Single-Channel GEN2 Pipette (P20)
  • 8-Channel Channel GEN2 Pipette (P20)
  • Custom Protocol Development


  • Temperature Module
  • Thermocycler Module (Optional)


  • Opentrons 20 uL Filter Tip Racks (9600 tips)
  • 4-in-1 Tube Rack Set
  • Opentrons Tough PCR Plates (2 x 25 count)
  • NEST 12-Well Reservoirs, 15 mL (50 count)
  • NEST 2.0 mL Microcentrifuge Tubes (500 count)

Frequently Asked Questions

For FAQs about the OT-2 and other products, click here.

Opentrons offers 2 purchasing options for our PCR Workstation. These two options are WITH a Thermocycler Module and WITHOUT a Thermocycler Module. All other components of the workstation are the same.

Configure your PCR Workstation with a Thermocycler Module for end-to-end PCR automation, or without a Thermocycler for automated PCR setup with off-deck thermocycling. If you have any questions on the option that would work best for your lab, one of our Automation Specialists would be happy to advise.

Our PCR Workstation can fully automate PCR workflows for up to 96 samples per run.

One of our Automation Specialists would be happy to walk through the set-up that would work best for your lab’s throughput requirements via a quick consultation.

Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.

You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.

You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!

If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.

Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.

Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.

The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated

Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.

Risk free 60-day demo

Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.