Opentrons logo
Keep your samples safe – learn more about our new HEPA/UV module

Protocol Library

Free plug-and-play automation workflows for Opentrons robots

Introducing the new Protocol Library

Unlock new possibilities for your lab



Verified protocols for Opentrons Flex and OT-2


Advanced search and filtering by categories


Personal user accounts to easily access custom protocols

Protocols for your application

The Protocol Library is an open, searchable repository of downloadable and configurable protocols for lab automation on Opentrons robots. Within the library, researchers can find protocols for a wide range of scientific workflows, including nucleic acid extraction, NGS library prep, protein purification, ELISA, and cell-based assays.

At the moment the Opentrons Protocol Library is probably the best collection of automated scripts you can find and with the Flex, we’ll be able to see that grow. A new engineer could learn a lot from just reading those scripts.

– Luis Villa, Senior Automation Engineer, Cellares

Verified by our scientists and partners

Verified protocols have undergone rigorous testing by Opentrons’ scientists and our reagent partners. The Opentrons Protocol Library fosters open science and collaborative validation of research.

Protocol Library V1

Find automated protocols for your application