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OT-2 Robot

The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.

The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.

Prices listed here are valid in the US and select other territories. Please get in touch with your local team for pricing for your territory.


1-Year Warranty

Ships in 28 business days

Create a quote after you add to cart
Create a quote after you add to cart


Choose your hardware and pipettes

11 deck slots, plus trash, and space for two swappable, single and 8-Channel pipettes. Magnetic, Temp, Thermocycler, Heater-Shaker, and HEPA Filter modules available.

Use your existing labware and reagents

Reagent and consumables agnostic. Compatible with any ANSI/SLAS-compliant labware.

Small Footprint, Big Impact

Fits on half a standard lab bench. Performs like robots costing 10x more (Accuracy: 15% at 1uL; Precision: 5% at 1uL).

Space for two pipettes

Reliable liquid transfers from 1–1000 µL with our swappable single- and 8-channel pipettes.

Automate the most time-consuming applications

The quickest way to save time in the lab. You can easily automate a range of basic liquid handling tasks on the OT-2 pipetting robot, including tube and microplate filling, serial dilution, cherry picking, normalization and sample pooling.

Tech Specs


63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H)
Takes up half of your standard lab bench


48 kg or 105.8 lb

Pipette configurations

Single- and 8-channel pipetting
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.

Pipette volumes and specs

Single-channel: 1-1000 µL
8-channel: 1-300 µL


11 deck slots enable countless configurations
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.


WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0


Integrated video camera (Included for future or custom functionality)


Integrated audio system (Included for future or custom functionality)

Power requirements

Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information, please review this support article

Frame composition

Rigid steel and CNC aluminum design

Operating temperature & relative humidity

Recommended 20-24 °C
Recommended 40-60% RH


Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights


For best practices on automating viscous liquid handling, review our application note

API compatibility

Interfaces with any product that has an API
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.


Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later.

Tip compatibility

Use Opentrons Tips
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.

Labware compatibility

Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.


One year warranty against manufacturer defects
Stress tested to last a minimum of 3 years of full-time use

Return policy

60 day, 100% risk free


Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)

Example Commands

Tip Handling

  • Pick up tip
  • Drop tip
  • Return tip
  • New tip (always, never, once)
  • More…

Liquid Control
  • Aspirate
  • Dispense
  • Aspirate speed
  • Dispense speed
  • Blowout
  • Touch tip
  • Mix
  • Air gap
What's in the box

OT-2 robot
Calibration block
Removable block
Trash container
Quickstart guide
Power supply and regional IEC cable
Ethernet cable
Ethernet-to-USB dongle
Tool kit


Manuals and White Papers
Certifications, Safety, and Compliance


Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.

You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.

You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!

If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.

Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.

Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.

The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated

Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.

Join a growing community

Thousands of labs in 40+ countries automate with Opentrons