Automating NGS Library Prep

NGS library preparation is complicated and has many steps, making automation a daunting prospect. With Opentrons robots and verified protocols, library preparation might become the easiest part of your day.

Download from a library of protocols

You don’t even need to program your robot to do your library preparation – that’s probably been done already. Simply visit the Opentrons Protocol Library and download protocols for kits from common NGS companies including Illumina, IDT, Qiagen and many more. Choose from Opentrons Verified or Manufacturer Verified protocols for immediate confidence, or Community Verified protocols to precisely replicate other scientists’ methods.

Protocol Library

Flex NGS Workstation

Save time by selecting the Flex NGS Workstation– everything you need to automate your workflow, so you can get to your science faster. With the Flex NGS Workstation, you can automate your workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time.

What’s Included

Key considerations for automating NGS library prep – with live demo

Automating NGS library prep is an important part of scaling genomics workflows, and offers several advantages, such as reduced hands-on time, increased consistency between samples and fewer errors. Join us for this webinar where we’ll outline some important considerations for automating NGS library prep, and then demo an NGS workflow on the Opentrons Flex NGS Workstation, a benchtop liquid handler with 96-sample throughput.

Watch the Webinar

Application Note

Ultra-efficient automated whole genome sequencing

A library prep workflow using QIAseq® FX DNA Library Kit and QIAseq® Normalizer Kit on the Opentrons Flex

Download App Note

Technical Bulletin

Opentrons Flex: Flexible automation for NGS

Platform overview and application data from NGS library prep, nucleic acid extraction and bead-based protein purification

Read Technical Bulletin

“

The Flex very materially helps us do library prep and saves time and mental energy. More importantly it changes how we think about things. How can we systematize this so it can be done more efficiently overall? I like that culture overall. It’s a much more high-performing way to do things.

– Peter McCaffrey, MD, FCAP, Assistant Professor: UTMB, CTO: Pragma Bio

Resources

Reagent	Application Data	Protocols	Verification *
QIAGEN QIAseq FX DNA Library Kit	Automating DNA Library Prep QIAseq FX plus Normalizer on Opentrons Flex	QIAseq FX DNA Library Kit on Opentrons Flex QIAseq FX DNA Library Kit with QIAseq Normalizer Workflow B on Opentrons Flex	Opentrons Partner
QIAGEN QIAseq Library Normalizer Kit	Automating DNA Library Prep QIAseq FX plus Normalizer on Opentrons Flex	QIAseq Library Normalizer Kit Workflow A on Opentrons Flex	Opentrons Partner
Illumina DNA Prep Kit	Automated library preparation of small genomes using DNA preparation kits on OT-2	Illumina DNA Prep on Opentrons Flex (coming soon) Illumina DNA Prep on OT-2	Opentrons
IDT xGEN DNA EZ and MC Library Prep Kits		IDT xGEN DNA EZ Library Prep Kit on Opentrons Flex IDT xGEN DNA EZ Library Prep Kit on OT-2 IDT xGEN DNA MC Library Prep Kit on OT-2	Opentrons
IDT xGEN Normalase Module		NGS Library Normalization with IDT xGEN Normalase Module on OT-2	Opentrons
seqWell ExpressPlex Library Prep Kit	[Poster] Automated, Multiplexed Preparation of up to 384 Plasmid Libraries Supports a 24-Hour End-to-End Sequencing Workflow	seqWell ExpressPlex Library Preparation on Opentrons Flex (2-part protocol)	Opentrons Partner
Roche KAPA HyperPrep Kit	Automated library preparation of small genomes using DNA preparation kits on OT-2	Roche KAPA HyperPrep Kit on Opentrons Flex (coming soon) Roche KAPA HyperPrep Kit on OT-2	Opentrons
NEBNext Ultra II DNA Library Prep Kit for Illumina	Automated library preparation of small genomes using DNA preparation kits on OT-2	NEBNext Ultra II DNA Library Prep Kit for Illumina on OT-2	Opentrons Partner
Nextera XT DNA Library Preparation Kit		Illumina Nextera XT on OT-2 (4-part protocol)	Community

*Verification categories:
Opentrons = has been verified by the Opentrons Team, who verify that the scientific results are as expected.
Partner = has been verified by our reagent partner, who verifies that the scientific results are as expected.
Community = protocol is in use by members of the Opentrons community.

Want to know more?

Our team of experts can help figure out if automation is right for you. Book a virtual demo to discuss your workflow needs with an expert.

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