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Opentrons Flex NGS Workstation

Flex NGS e1689988289771
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Opentrons Flex NGS Workstation


For scaling up and fully automating end-to-end NGS library prep

The Opentrons Flex NGS Workstation includes the Flex Robot, Pipettes, Tips, Labware, Thermocycler, Magnetic Block, Temperature Module, and Custom Protocol Development.

With the Flex NGS Workstation, you can automate your NGS workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. The Flex enables you to automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.

The Flex NGS Workstation can support the following workflow steps:


Library Construction


Bead Clean Ups


Normalization and Pooling


Library & Sample Prep

Well Plates

Tagmentation & Transformation

What’s in the box

Each Flex NGS Workstation is configured with hardware, consumables, and custom protocol development for your NGS assay.

RobotsFLGNS 1

Robot & Pipettes

  • Opentrons Flex Robot
  • Flex Gripper
  • Flex 8-Channel Pipette (1-50 µL)
  • Flex 8-Channel Pipette (5-1000 µL)
  • Custom Protocol Development
ModulesFLNGS 1


  • Flex Magnetic Block
  • Temperature Module
  • Thermocycler Module
  • Heater-Shaker Module (Optional)
LabwareFLNGS 1

Accessories & Labware

  • Flex Waste Chute
  • Flex Deck Expansion Set (Optional)
  • Flex Pipette Tips 50 µL, Filtered (80 Racks/20 Refills)
  • Flex Pipette Tips 200 µL, Filtered (180 Racks/20 Refills)
  • NEST 12-Well Reservoirs (50 Count)
  • Tough 0.2 mL 96-Well PCR Plates (2 x 25 Count)
  • NEST 2.0 mL Microcentrifuge Tubes (500 Count)
  • 4-in-1 Tube Rack Set

Frequently Asked Questions

For FAQs about the OT-2 and Opentrons Flex, click here.

Yes, this can be achieved through the Opentrons app. You will need to be connected to a computer that is on the same WiFi or wired network as the liquid handler.

This is not an optical process. The robot uses capacitance sensors and a calibration probe (shipped with Flex pipettes) to perform automated calibration. Capacitive sensing for automated calibration is done by having a current run through the pipette’s metal tip at the bottom and then through a calibration tip until it’s grounded.

The Opentrons Flex 8-channel and 96-channel pipettes will aspirate and dispense the same volume with all channels at the same time. Volume is not independently controlled across channels.

OT-2 pipettes and OT-2 pipette tips are not compatible with the Opentrons Flex. The OT-2 pipette nose cone and Flex pipette nose cones are constructed differently, preventing cross-compatibility. OT-2 tip boxes/refills are also not compatible with the Flex.

Opentrons can only guarantee performance of the Flex when using our Flex pipette tips.

Flex Fits You

Find out more about the Opentrons Flex from an automation expert