Proteomics throughput is increasing, driven by advances in resolution, cost, and speed. Manual pipetting during sample preparation not only wastes time, but has become a bottleneck limiting research, leading labs all over the world to embrace automation.
The Opentrons Flex Absorbance Plate Reader Module is a fully automated solution for absorbance-based assays. Using the example of protein quantification by BCA assay, our scientists will show how to use an Opentrons Flex with the newly released on-deck absorbance plate reader to set up and run assays, and then normalize concentrations, all from a single protocol without human intervention.
Check out this article in Genetic Engineering and Biotechnology News discussing how sample preparation has become a bottleneck limiting proteomics research, and how the use of liquid handling automation has overcome this problem, enabling researchers to focus on their science.
Watch our on-demand demonstration of how the Opentrons Flex and its modules automate complex proteomics tasks, and get tips on how your day-to-day protein sample processing work can be seamlessly performed on the platform.
With the Flex Magnetic Bead Protein Purification Workstation, you can automate your bead-based protein purification workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate small-scale isolation of specific proteins, protein complexes, and antibodies, or proteomics sample processing, using many leading magnetic bead-based reagent systems on the market.
Save time by downloading free protocols from the Opentrons Protocol Library. Select from a range of protein extraction (including SP3), quantification, and proteomic sample preparation methods including digestion and normalization.
An end-to-end solution that provides a cost-effective workflow for high-throughput sample processing, encompassing protein concentration determination and sample digestion.
Automated Protein Quantification Assays for the Opentrons Flex
Automation of three ELISA protocols on the Opentrons OT-2 with an on-deck Heater-Shaker Module
Automation of bead-based ELISA forSARS-CoV-2 neutralizing antibody on the Opentrons OT-2 with an on-deck Heater-Shaker Module
Automated Protein Purification with Biotage Dual Flow Chromatography PhyTip Columns on the OT-2
Automated Ni-NTA bead-based protein purification on the OT-2 liquid handler
A Semiautomated Paramagnetic Bead-Based Platform For Isobaric Tag Sample Preparation