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Get started: Python API onboarding protocol tutorial

Try out a basic protocol from the Opentrons Protocol Library to get started using the Python API with your OT-2. Read More

Get started: Power on the OT-2

Learn how to connect the power supply and turn on your OT-2. Read More

Get started: Install the top window and the window covers

Learn how to install the top window and the removable covers for the holes in the left, right, and rear windows on your OT-2 to reduce cross-contamination. Read More

Get started: Import a protocol

After you’ve attached pipettes and finished deck, tip length, and pipette offset calibrations, you can import a protocol to the Opentrons App. Read More

Get started: Download and install the Opentrons App

Learn how to download and install the Opentrons App to connect to your robot. Read More

Get started: Connect to your OT-2 over Wi-Fi (optional)

Learn how to connect to your OT-2 over Wi-Fi to run protocols wirelessly, from anywhere—as long as your computer is on the same network as the OT-2. Read More

Get started: Connect to your OT-2 over USB

Learn how to connect to your OT-2 using the USB A/B cable included in your order. Read More

Get started: Choose which protocol method is right for you

Choose between Protocol Designer, which is best for basic workflows and requires no coding, or Python API, which offers unlimited control and customization and requires Python proficiency. Read More

Get started: Check what’s included with your OT-2

See which parts and accessories included in OT-2 robot orders. These include tools required for setup, spare parts for common maintenance and repair, and accessories that can be used to attach labware to the robot. Read More

Get started: Calibrate tip length and pipette offset

Learn how to perform tip length and pipette offset calibration for each pipette attached to your OT-2. Read More