Lab automation has never been easier

The high-precision OT-2 lab robot is flexible, open-source, and user-friendly.
Ideal for:
  • Genomics
  • Proteomics
  • Cell-based assays
  • Biochemical assays
  • Drug discovery
View the OT-2 Video
flexible hardware

One robot, countless workflows

  • Configurable 11-deck platform with options for Magnetic, Temperature and Thermocycler Modules
  • Swappable single & 8-channel pipettes for multiple volume configurations
  • Standardized (SBS) deck slots allow you to use labware you already own


  • Accuracy: 15% at 1uL
  • Precision: 5% at 1uL
  • Throughput: Fast fill a 96-well plate in 22 seconds
flexible software

Powerful software at your fingertips

  • Choose from free, beginner-friendly & advanced protocol controls
  • Powerful cloud-based tools
  • Unmatched control of nearly every aspect of your workflow

User Friendly

  • Self-serve unboxing and installation
  • Reagent and bead system agnostic
  • Plug-and-play setup with access to protocol libraries to enable immediate assay implementation
  • Easily integrates to your existing instruments
  • Compatible with ANSI/SBS compliant or automation friendly tubes, microplates, deep-well plates, reservoirs and adapters
open source

Near limitless control

  • 100% customizable and open to modifications adjustable to your assay
  • Modify or customize protocols with our simple yet powerful Python API
  • Digital protocol commands surpass manual pipetting capabilities


  • Supports Ethernet or USB connection (external dongle provided)
  • 4 USB ports available to support connection to multiple modules
  • Easy access to electronics via external PCBA enclosure
  • LED indicator provide users feedback, diagnosis & troubleshooting aid on instrument power state, system processing, ethernet, and network connectivity

So easy, you’ll feel like an automation expert

Real-time chat support on your computer or mobile phone.

Plug-and-play. Unbox, set-up, calibrate. No technician needed.

Dedicated Account Managers available to you at every step.

Start with confidence. Demo the OT-2 for 60 days.


Lab automation rethought from the ground up

OT-2 Tech Specs Overview
OT-2 Deck Outline
  • Compact footprint
  • 63cm x 57cm x 66cm / 25in x 22.5in x 26in (W,D,H)
  • Takes up half of your standard lab bench
  • Pipette configurations
  • Single and 8-channel pipetting
  • 2-pipette mounts, for a configuration of 1 or 2 single or 8-channel pipettes. Pipettes are easily interchangeable.
  • Deck
  • 11 deck slots enable countless configurations
  • Deck slots are compatible with standard SBS dimensions. Deck also includes a removable trash bin.
  • Weight
  • 48kg or 105.8 lb
  • Video
  • Integrated video camera (Included for future or custom functionality)
  • Audio
  • Integrated audio system (Included for future or custom functionality)
  • Power Requirements
  • Robot Power Input: 36VDC, 6.1A
  • Power Adapter Input: 100-240VAC, 50/60Hz, 4.0A/115VAC, 2.0A/230VAC
  • For additional power requirement information, please review this support article
  • Frame composition
  • Rigid steel and CNC aluminum design
  • Operating Temperature & Relative Humidity
  • Recommended 20-24C Recommended 40-60% RH


Fills a 96-well plate in 22 seconds

Time to fill 96-well plate
One Tip
Same Tip
New Tip
P20 Single
P300 Single
P1000 Single

Accuracy and precision

Single and 8-channel pipetting

Learn more ›

Single Channel Accuracy & Precision
PipetteVolumeRandom ErrorSystematic Error
p20 1±15%0.15µL±5%0.05µL
p300 20±4%0.8µL±2.5%0.5µL
p1000 100±2%2.0µL±1%1µL
8-Channel Accuracy & Precision
PipetteVolumeRandom ErrorSystematic Error
P20 1±20%0.2µL±10%0.1µL
20± 2.2%0.44µL±1.5%0.3µL
P300 20±10%2.0µL±4%0.8µL


Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment

Add the Opentrons HEPA Module, or add your own UV lights


For best practices on automating viscous liquid handling, review our application note

Viscous Liquid Handling Automation using Opentrons OT-2

  • API compatibility
  • Interfaces with any product that has an API
  • Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
  • Tip compatibility
  • Use Opentrons Tips and Opentrons Filter Tips
  • Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
  • Labware compatibility
  • Use common labware already in our Labware Library, or add your own
  • If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
  • Warranty
  • One year warranty against manufacturer defects
  • Stress tested to last a minimum of 3 years of full-time use
  • Certifications
  • Certifications complete (CE, FCC)
  • Certifications pending (NRTL, CB)
  • Not certified/validated (IVD, GMP, ISO)
Tip Handling
  • Pick up tip
  • Drop tip
  • Return tip
  • New tip (always, never, once)
  • More...
Liquid Control
  • Aspirate
  • Dispense
  • Aspirate speed
  • Dispense speed
  • Blowout
  • Touch tip
  • Mix
  • Air gap
  • Move to
  • Delay
  • User-specified pause
  • Homing
  • Comment
  • Basic transfer
  • Volumes > Pipette Volume
  • Distribute
  • Consolidate
Variable Settings
  • Speed
  • Volume
  • Count
  • Dispose

Welcome To Opentrons!

Welcome To Opentrons Video

How The Opentrons Platform Works

How The Opentrons Platform Works Video

Custom Modular Hardware

Custom Modular Hardware Video

Setting Up Your OT-2

Setting Up Your OT-2 Video

OT-2 Hardware Video

View the OT-2 Hardware Video

Risk free 60-day demo

Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.