The OT-2 is a bench-top liquid handler designed to be accessible and flexible enough to automate many common applications.
Our life is so much easier now because the OT-2 is really good.
– Marilene Pavan, Boston University
There are days we have Opentrons transfer 800 samples. I would never be able to do as many samples by hand.
– Kaja Wasik, Gencove
Before, I had to leave one whole day for one ELISA run. Now, the robot frees my hands.
– Annabel Shang, MilliporeSigma
We are currently working with our finance folks to acquire two additional OT-2 instruments, because we LOVE this platform.
– Adam Ericsen, Emory University
One of the most common applications for automation with the OT-2 pipetting robot. Not only can you automate NGS library prep, but also other steps of your pre-sequencing workflow, including reagent distribution, bead processing, normalization and pooling. Use your own protocol, or choose from demonstrated protocols with popular reagent brands.
Another popular application for automation, especially if you’re prepping a lot of samples. You can use the OT-2 pipetting robot to combine samples and PCR mastermix in the reaction plate that will go into the thermocycler or qPCR machine.
The most cost-effective way to automate small-scale protein purification, proteomics sample processing, and enzymatic and chemical lysis. We currently offer two approaches to automate on the OT-2 pipetting robot – dual flow chromatography-based automation, and magnetic bead-based automation.
An easy and cost-effective way to automate tedious nucleic acid purifications. If you are using SPRI (solid-phase reversible immobilization) magnetic beads to do your isolation and/or purification steps, you can automate these parts of your workflow with the OT-2 pipetting robot.
The quickest way to save time in the lab. You can easily automate a range of basic liquid handling tasks on the OT-2 pipetting robot, including tube and microplate filling, serial dilution, cherry picking, normalization and sample pooling. Choose from a range of verified protocols in our Protocol Library, create your own, or ask our Applications Engineering team to create one for you
Everything you need to automate your lab, including support at every step
Demo the OT-2
Get a virtual demo of the OT-2 and get answers about your specific application
Order the OT-2
Bundle your OT-2 with the pipettes, hardware and labware you need
Receive and unbox your OT-2
Easy to unbox and set up yourself using our online guide
Custom or urgent support?
Contact us for onsite installation, custom protocol development and more!
11 deck slots, plus trash, and space for two swappable, single and 8-Channel pipettes
Magnetic, Temp, Thermocycler, Heater-Shaker, and HEPA Filter modules available
Reagent and consumables agnostic
Compatible with any ANSI/SLAS-compliant labware
A protocol is the code that runs the robot through your experiment. We’ve built different ways to create a protocol that are accessible for everyone – from the automation rookie to the experienced Python coder.
Best for users that want a unique or complex protocol – fast
Get our experts to create a protocol optimized for your experiment
Best for new users looking to gain experience
Browse well-documented, open-source protocols
Best for users who want to create a new protocol – without having to code
Use our intuitive drag-and-drop interface to create new protocols
Best for users with Python experience
Write your own protocol using our open-source Python Protocol API
Reliable liquid transfers from 1–1000 µL with our swappable single- and 8-channel pipettes
On-call support offers real-time chat support on your desktop or mobile
Visit the Knowledge Hub to Browse our in-depth support articles and videos
Have our experts develop a Custom Protocol for your application
Warranties and services maximize your lab’s performance
63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H)
Takes up half of your standard lab bench
48 kg or 105.8 lb
Single- and 8-channel pipetting
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.
11 deck slots enable countless configurations
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.
WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
Integrated video camera (Included for future or custom functionality)
Integrated audio system (Included for future or custom functionality)
Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information, please review this support article
Rigid steel and CNC aluminum design
Recommended 20-24 °C
Recommended 40-60% RH
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Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights
Interfaces with any product that has an API
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
Use Opentrons Tips and Opentrons Filter Tips
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
One year warranty against manufacturer defects
Stress tested to last a minimum of 3 years of full-time use
Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)
Power supply and regional IEC cable
To view FAQs for all products, click here.
You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!
If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.
Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.
Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.
The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated
Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.
Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.