End-to-end NGS library prep of up to 24 samples
The NGS Workstation includes OT-2 Robot, Pipettes, Tips, Labware, Thermocycler, Magnetic and Temperature Modules, and Custom Protocol Development.
With the OT-2 NGS Workstation, you can automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.
The OT-2 NGS Workstation can support the following workflow steps:
Bead Clean Ups
Normalization and Pooling
Library & Sample Prep
Tagmentation & Transformation
Each OT-2 NGS Workstation is configured with hardware, consumables, and custom protocol development for your NGS assay.
Automated library preparation of small genomes using DNA preparation kits (Illumina DNA Prep Kit, KAPA HyperPrep kit, and NEBNext Ultra II Kit)
Fully Automated NGS Clean-up and Size Selection with Omega Bio-Tek Mag-Bind® TOTALPURE NGS on the OT-2
Robust and high-throughput SARS-CoV-2 viral RNA detection, research, and sequencing using RNAdvance Viral and the OT-2 platform
NGS Library Prep with Roche HyperPrep Kit
NGS Library Prep with NEBNext Ultra II DNA Library Prep Kit for Illumina
NGS Library Prep with IDT xGEN DNA Library Prep EZ Kit
NGS Library Prep with IDT xGEN DNA Library Prep MC Kit
NGS Library Normalization with IDT xGEN Normalase Module
NGS Cleanup and Size Selection with Omega Bio-tek Mag-Bind® TotalPure NGS
NGS Cleanup with MACHEREY-NAGEL NucleoMag NGS Clean-Up
NGS Size Selection with MACHEREY-NAGEL NucleoMag NGS Double-Size Selection
For FAQs about the OT-2 and other products, click here.
We’ve validated protocols for:
Omega Bio-Tek’s Mag-Bind TotalPure NGS
You can browse all of our community’s NGS Library Prep protocols within our Protocol Library. Don’t see a protocol for the kit your lab is using? We offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.
Our NGS Workstation can fully automate library preparation for up to 24 samples per run. Increased throughputs are possible, however manual steps (e.g. swapping out tip racks) would be required.
One of our Automation Specialists would be happy to walk through the set-up that would work best for your lab’s throughput requirements via a quick consultation.
Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.
You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.
You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!
If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.
Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.
Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.
The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated
Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.
Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.