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Knowledge Hub


So you want to automate PCR? Webinar

This webinar will review and recommend strategies for automating PCR + PCR prep with the Opentrons OT-2 and Opentrons Flex.


Automated PCR with an On-deck Thermocycler Webinar

Interested in automating your endpoint PCR with an on-deck thermocycler? This webinar will introduce you to our next-generation Opentrons Thermocycler…

Documentation & Manuals

Thermocycler Module Quickstart Guide

The Opentrons Thermocycler Module GEN2 provides ondeck, fully automated thermocycling. The module enablesautomation of upstream and downstream workflow steps…


Automating PCR eBook

PCR is a molecular biology workhorse that is used for detecting and quantifying specific genes and is an important component…


How To Save Time In The Lab With An Automated On-Deck Thermocycler

The difference between using an automated thermocycler on- and off- the deck of your liquid handler is reclaimed time.


Custom CSV Transfer Protocol

Accomplish complex workflows, easily with this Custom CSV Transfer Protocol. This protocol utilizes a CSV to specify aspiration height, labware,…


Plate Reformatting

Our most robust cherrypicking protocol. Specify aspiration height, labware, pipette, as well as source and destination wells with this all…


Generic PCR Prep

This protocol allows your robot to create a master mix solution using any reagents stored in one to three different…

Application Notes

Automating Sensitive Polymerase Chain Reaction Protocols Using the Opentrons Thermocycler on the Opentrons OT-2 Platform

There is an increase in demand for sensitive polymerase chain reaction (PCR) techniques to be automated. In this application note,…

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