Get started automating your lab today
The OT-2 is a bench-top liquid handler designed to be accessible and flexible enough to automate many common applications.
Our life is so much easier now because the OT-2 is really good.
– Marilene Pavan, Boston University
There are days we have Opentrons transfer 800 samples. I would never be able to do as many samples by hand.
– Kaja Wasik, Gencove
Before, I had to leave one whole day for one ELISA run. Now, the robot frees my hands.
– Annabel Shang, MilliporeSigma
We are currently working with our finance folks to acquire two additional OT-2 instruments, because we LOVE this platform.
– Adam Ericsen, Emory University
Everything you need to automate your lab, including support at every step
Demo the OT-2
Get a virtual demo of the OT-2 and get answers about your specific application
Order the OT-2
Bundle your OT-2 with the pipettes, hardware and labware you need
Receive and unbox your OT-2
Easy to unbox and set up yourself using our online guide
Custom or urgent support?
Contact us for onsite installation, custom protocol development and more!
11 deck slots, plus trash, and space for two swappable, single and 8-Channel pipettes
Magnetic, Temp, Thermocycler, Heater-Shaker, and HEPA Filter modules available
Reagent and consumables agnostic
Compatible with any ANSI/SLAS-compliant labware
A protocol is the code that runs the robot through your experiment. We’ve built different ways to create a protocol that are accessible for everyone – from the automation rookie to the experienced Python coder.
Best for users that want a unique or complex protocol – fast
Get our experts to create a protocol optimized for your experiment
Best for new users looking to gain experience
Browse well-documented, open-source protocols
Best for users who want to create a new protocol – without having to code
Use our intuitive drag-and-drop interface to create new protocols
Best for users with Python experience
Write your own protocol using our open-source Python Protocol API
Reliable liquid transfers from 1–1000 µL with our swappable single- and 8-channel pipettes
P20
P300
P1000
P20
P300
On-call support offers real-time chat support on your desktop or mobile
Visit the Knowledge Hub to Browse our in-depth support articles and videos
Have our experts develop a Custom Protocol for your application
Warranties and services maximize your lab’s performance
63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H)
Takes up half of your standard lab bench
48 kg or 105.8 lb
Single- and 8-channel pipetting
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.
Single-channel: 1-1000 µL
8-channel: 1-300 µL
11 deck slots enable countless configurations
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.
WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
Integrated video camera (Included for future or custom functionality)
Integrated audio system (Included for future or custom functionality)
Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information, please review this support article
Rigid steel and CNC aluminum design
Recommended 20-24 °C
Recommended 40-60% RH
| Pipette | Distribute, one tip |
Transfer, same tip |
Transfer, new tip |
|---|---|---|---|
| P20 8-Channel | 0:22 | 1:35 | 4:19 |
| P300 8-Channel | 0:26 | 1:38 | 4:24 |
| P20 Single-Channel | 3:12 | 13:10 | 36:01 |
| P300 Single-Channel | 4:01 | 13:39 | 41:31 |
| P1000 | 5:54 | 15:21 | 44:50 |
| Pipette | Volume | Random Error | Systematic Error | ||||
|---|---|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||||
| P20 | 1 10 20 |
±15% ±2% ±1.5% |
0.15µl 0.2µl 0.3µl |
±5% ±1% ±0.8% |
0.05µl 0.1µl 0.16µl |
||
| P300 | 20 150 300 |
±4% ±1% ±0.6% |
0.8µl 1.5µl 1.8µl |
±2.5% ±0.4% ±0.3% |
0.05µl 0.6µl 0.9µl |
||
| P1000 | 100 500 1000 |
±2% ±1% ±0.7% |
2.0µl 5.0µl 7.0µl |
±1% ±0.2% ±0.15% |
1µl 1µl 1.5µl |
| Pipette | Volume | Random Error | Systematic Error | ||||
|---|---|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||||
| P20 | 1 10 20 |
±20% ±3% ±2.2% |
0.2µl 0.3µl 0.44µl |
±10% ±2% ±1.5% |
0.1µl 0.2µl 0.3µl |
||
| P300 | 20 150 300 |
±10% ±2.5% ±1.5% |
2.0µl 3.75µl 4.5µl |
±4% ±0.8% ±0.5% |
0.8µl 1.2µl 1.5µl |
Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights
Interfaces with any product that has an API
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
Use Opentrons Tips and Opentrons Filter Tips
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
One year warranty against manufacturer defects
Stress tested to last a minimum of 3 years of full-time use
Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)
Tip Handling
Utility
Transfer
Variable Settings
Other
OT-2 robot
Calibration block
Removable block
Trash container
Quickstart guide
Power supply and regional IEC cable
Ethernet cable
Ethernet-to-USB dongle
Tool kit
To view FAQs for all products, click here.
Yes, the Opentrons Flex’s side windows and front door are removable.
The Heater-Shaker Module can assist in automating a variety of wetlab workflows. Here is an example of a DNA Extraction protocol leveraging the Heater-Shaker Module.
Yes, this can be achieved through the Opentrons app. You will need to be connected to a computer that is on the same WiFi or wired network as the liquid handler.
Yes. Just like the OT-2, the Flex has full capability to accept custom or irregular labware. Our custom labware creation tools can be used to help create the compatible labware definitions that are required to use irregular labware with our software.
It is possible to use multiple modules of the same type on the OT-2. This is true for our Temperature Module, Magnetic Module, and Heater-Shaker Module. For a walkthrough on setting this up for your OT-2 and considerations when using multiple modules, please reference this guide.
You can use one computer running the Opentrons App to connect to multiple robots. This is true for both the Opentrons Flex and OT-2 robots.
OT-2 pipettes and OT-2 pipette tips are not compatible with the Opentrons Flex. The OT-2 pipette nose cone and Flex pipette nose cones are constructed differently, preventing cross-compatibility. OT-2 tip boxes/refills are also not compatible with the Flex.
Opentrons can only guarantee performance of the Flex when using our Flex pipette tips.
No, Opentrons’ OT-2 pipettes will not be compatible with the Opentrons Flex. The Opentrons Flex is intended for use with our Flex Pipettes.
Yes, Flex pipettes include pressure sensors for detecting liquid height. These sensors are contained within the A1 and H12 channels on the 96-channel pipette, and the outermost channels on the 8-channel pipette (1 & 8).
Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.
Users do not need a computer connected to the Opentrons Flex to run a protocol. Users will need to build the protocol on a computer and upload it to the Flex. Once uploaded, the protocol can be ran solely off the display.
You do not need to know how to code to use the Opentrons Flex platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.
You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.
The Opentrons Flex 8-channel and 96-channel pipettes will aspirate and dispense the same volume with all channels at the same time. Volume is not independently controlled across channels.
Yes, Opentrons Flex users will be able to offset the 96-channel pipette head to pick up 8 tips (1 column). This functionality can only be achieved through the Python API.
Crashes will be detected by the Opentrons Flex by comparing the actual vs. intended move of the embedded motors.
For example, If the robot expected 2 motor rotations, but only 1 rotation is completed due to a crash(which stops the motors from moving), then the robot would detect a discrepancy between intended move and actual. This would then throw an error and let you know you need to recalibrate.
Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.
Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.
Yes, we are still using stepper motors with linear encoders. This enables us to track the steps of the motor and handle errors with positioning.
For those familiar with the homing patterns of the OT-2 robot, we no longer rely on rehoming the axis with limit switches in an open-looped feedback architecture. We can use a closed-loop system, powered by our new encoders to reduce the number of times that we have to home in a protocol.
This is not an optical process. The robot uses capacitance sensors and a calibration probe (shipped with Flex pipettes) to perform automated calibration. Capacitive sensing for automated calibration is done by having a current run through the pipette’s metal tip at the bottom and then through a calibration tip until it’s grounded.
Users can save up to 8 protocols to the Opentrons Flex display.
To pick up tips, The Opentrons Flex 96-Channel Pipette requires tip racks be in a 96-Channel Tip Rack Adapter. Four 96-Channel Tip Rack Adapters are included with the purchase of one Flex 96-Channel Pipette.
Yes! When carrying out multiple protocols on a Heater-Shaker Module, it may be required to have the module heat, but not shake. This option is possible on the Heater-Shaker Module.
The Opentrons Flex is not currently CFR 21 Part 11 compliant. Opentrons is in the process of pursuing CFR Part 11 compliance for the Flex.
Yes. Compatible labware for the Opentrons Flex Magnetic Block includes:
The Opentrons Flex requires on-site installation. We also offer both remote and on-site services solutions, as well as annual preventative maintenance.
You can find more information on Opentrons’ service offerings here.
The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated
You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!
If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.
The Opentrons Flex Magnetic Block GEN1 is compatible with the Opentrons Flex robot, while the OT-2 Magnetic Module GEN2 is compatible with the OT-2 robot.
The Magnetic Block is a plate-sized magnet that relies on the Opentrons Flex Gripper GEN1 to move your plates on and off the block. Conversely, the Magnetic Module is a powered unit that turns the magnets on and off, and is intended for use with a liquid handling robot without a gripper.
20 cm (8 in) of side and back clearance for cables, USB connections, and to dissipate exhaust from instruments like the Thermocycler or Temperature Module.
Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.
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