These troubleshooting steps address issues such as tips unexpectedly dropping, pipette not aspirating properly, and uneven aspiration across channels of a multi-channel pipette. Read More

If you’re unable to establish a Wi-Fi connection to the OT-2, follow the steps outlined in this article. Read More

If your OT-2 is having difficulty communicating with the attached pipette, ensure the white connector tab is securely plugged into the pipette and recheck the connection. Read More

If your OT-2 isn’t appearing in the Opentrons App despite being connected via USB, use this guide to troubleshoot common connection issues. Read More

There are two types of calibration-related technical issues: those hindering calibration processes and those affecting the robot’s movement during a protocol run. Read More

Opentrons Support might ask you to follow these specific instructions if you’re unable to update your OT-2 to the latest software version to benefit from the newest calibration features and bug fixes or if the usual troubleshooting methods haven’t helped. Read More

Outdated OT-2 robot software may inaccurately identify some protocols as empty. Update both your Opentrons App and robot software to the latest version to resolve this issue. Read More

A SmoothieError is how the OT-2 reports something that went wrong with a motor controller command. It is usually a symptom of an underlying mechanical or electrical problem. Read More

A homing fail occurs when the limit switch was unexpectedly not hit while the OT-2 was homing. Homing fails usually happen if a) you’re using different hardware with a different axis length or b) the axis is stuck or skipping during the home. Read More

A hard limit occurs when a limit switch was unexpectedly hit while the OT-2 was not homing. Essentially, it is when an endstop triggers when it is not supposed to (e.g. outside of a home). The error message will mention the axis that failed, like “hard limit +X.”… Read More