In Protocol Designer, you can instruct the robot on when to acquire a new tip in any step involving a pipette. Note that the available choices are limited based on the type of Transfer you are configuring. Read More

In Protocol Designer, batch edit mode enables you to edit multiple Transfer or Mix steps simultaneously, streamlining your protocol editing process. Read More

In Protocol Designer, air gap allows you to aspirate air into the tip after aspirating liquid from a source well. It’s typically used to prevent liquid from dripping out of the tip. Air gap can look a little different depending on the Transfer path you have selected. Read More

In Protocol Designer, both Mix and Transfer pipetting steps have advanced settings. Use the gear across from Aspirate or Dispense headers to access these settings. Read More

Protocol Designer enables the creation of simple pipetting protocols for Opentrons robots without the need for coding. Read More

In Protocol Designer, if a well contains an unnamed or “unknown” liquid, it indicates that the pipette has aspirated more than the volume of a source well. Read More

This article offers practical recommendations for effectively managing viscous liquids within Protocol Designer protocols, focusing on optimizing liquid handling techniques. Read More

This is a known limitation in software versions earlier than 4.0.0. During a protocol run, when the pipettes move to labware, you might notice one pipette goes to the correct place and the other pipette is up to 1-2 mm off, and that error is consistent throughout the protocol. Read More

The OT-2 doesn’t have embedded motors so it defaults to the bottom of the labware it is aspirating and dispensing from if you don’t tell it to do otherwise. This article reviews how to change the aspiration/dispense height in the Python API and Protocol Designer if you are aspirating from… Read More

When creating a protocol using 8-Channel pipettes and modules, there are certain deck layouts that will cause the pipette to crash into your modules. To prevent these crashes, you will want to avoid placing labware (including tip racks) requiring 8-Channel access directly adjacent to your modules. Read More