If your OT-2 isn’t appearing in the Opentrons App despite being connected via USB, use this guide to troubleshoot common connection issues. Read More

There are two types of calibration-related technical issues: those hindering calibration processes and those affecting the robot’s movement during a protocol run. Read More

Opentrons Support might ask you to follow these specific instructions if you’re unable to update your OT-2 to the latest software version to benefit from the newest calibration features and bug fixes or if the usual troubleshooting methods haven’t helped. Read More

Outdated OT-2 robot software may inaccurately identify some protocols as empty. Update both your Opentrons App and robot software to the latest version to resolve this issue. Read More

A SmoothieError is how the OT-2 reports something that went wrong with a motor controller command. It is usually a symptom of an underlying mechanical or electrical problem. Read More

A homing fail occurs when the limit switch was unexpectedly not hit while the OT-2 was homing. Homing fails usually happen if a) you’re using different hardware with a different axis length or b) the axis is stuck or skipping during the home. Read More

A hard limit occurs when a limit switch was unexpectedly hit while the OT-2 was not homing. Essentially, it is when an endstop triggers when it is not supposed to (e.g. outside of a home). The error message will mention the axis that failed, like “hard limit +X.”… Read More

This article describes how to obtain the information required to troubleshoot connection issues on a Windows 10 machine. Read More

Encountering this error on your OT-2 prevents protocol uploads and runs. Resolve it by updating the Opentrons App and OT-2 robot software to the latest version and calibrating the deck. Read More

Occasionally, the white ribbon cable connecting to the pipettes may get damaged and need replacement. Follow these instructions to remove the current ribbon cable from the OT-2 and attach the replacement. Read More