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Common errors when running opentrons_execute

This article provides troubleshooting instructions for specific errors you might encounter while running protocols outside the Opentrons App, such as through SSH or Jupyter Notebook. Read More

Accessing the OT-2’s rear electronics

On the back of the OT-2 and OT-2R robots, there are some electronics that are normally concealed by a black cover. If you’re working with Opentrons Support to troubleshoot a problem, we might ask you to remove the cover and expose the electronics. This article explains how to do this… Read More

Accessing the OT-2’s motor controller board

The motor controller board is responsible for electrically driving the motors that move the pipettes around the OT-2 and OT-2R robots. If you’re working with Opentrons Support to resolve certain technical problems, we might ask you to inspect or replace it. This guide will provide step-by-step instructions for removing the… Read More

8-Channel pipette rotated relative to labware

This article provides guidance on troubleshooting an issue with 8-Channel pipettes that appear visibly rotated around their vertical axis after moving to labware. Read More

Why does my 8-Channel pipette pick up tips so forcefully?

If you’re using an 8-Channel pipette, you might see it pick up tips with seemingly excessive force. That force is necessary for reliable tip pickup and seal. Read More

What are the maximum and minimum flow rates of the pipette?

This article lists the ranges of flow rates for GEN2 pipettes used on the OT-2. Read More

Using fewer than 8 channels of a multi-channel pipette

8-Channel GEN2 pipettes on the OT-2 allow you to utilize any number of channels ranging from one to eight during liquid handling steps for transfers and other actions. Read More

Realigning the OT-2 Z-stage carriage

This article explains how to adjust the Z-stage carriage of the OT-2 to correct pipette misalignment. Read More

Preparing older OT-2s for GEN2 pipettes

OT-2 robots that were manufactured before 2019-09-05 need a hardware adjustment before you can attach GEN2 pipettes to them. Read More

Pipette volumetric verification and calibration

This article provides information on performance verification of Opentrons GEN2 pipettes used on the OT-2. Read More