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HEPA Filter Module


The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.

HERO HEPA Assembled e1674231534813
HEPA Exploded 1
FRONT HEPA Isolated 1
FRONT HEPA Assembled
HERO HEPA Assembled
HERO HEPA Isolated 1


  • Reduces contamination risk during sensitive workflows
  • Vertical airflow downflow of 0.5 m/s – 1.0 m/s
  • 3 year fan lifetime of moderate use
  • 1 year filter lifetime of moderate use
  • Replaceable H14 HEPA filter
  • Compatible with all other OT-2 hardware modules

Tech Specs

HERO HEPA Assembled

Vertical downflow; 0.5 m/s – 1.0 m/s

HEPA filter grade/efficiency

99.99% efficiency for 0.3 um particles


On/off; Fan speed

Fan lifetime

3 years of moderate use

Filter lifetime

1 year of moderate use


625 x 599 x 303 mm (l/w/h)


60 lbs / 27 kg


110V – 220V

Noise level

65 decibels


Example Protocols
White Papers

Frequently Asked Questions

For FAQs about the OT-2 and other products, click here.

The HEPA Module is not a biosafety cabinet, nor does it protect the user. The module is used to maintain a sterile working environment inside the OT-2.

The HEPA Module should never be used for pathogens. It should not be used for situations where there are volatiles, or any potentially hazardous aerosolized materials.

The HEPA Module uses positive pressure to provide a sterile working environment inside the OT-2, as shown in the illustration on page 2 of our HEPA Module Whitepaper.

The Opentrons HEPA Module is intended for use with our OT-2 robot, and can not be used as a standalone unit.

Risk free 60-day demo

Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.