For scaling up and fully automating PCR setup and thermocycling
The Opentrons Flex PCR Workstation includes the Flex Robot, Pipettes, Tips, Labware, Temperature Module, optional Thermocycler, and Custom Protocol Development.
With the Flex PCR Workstation, you can automate your PCR setup and thermocycling at the scale you need to increase efficiency, reduce errors, and save hands-on time. Configure your workstation with our on-deck Thermocycler for end-to-end automation, or with no Thermocycler for automated PCR setup with off-deck thermocycling.
The Flex PCR Workstation can support the following workflow steps:
PCR Setup
Thermocycling
Each Flex PCR Workstation is configured with hardware, consumables, and custom protocol development for your PCR assay.
For FAQs about the OT-2 and Opentrons Flex, click here.
Yes, this can be achieved through the Opentrons app. You will need to be connected to a computer that is on the same WiFi or wired network as the liquid handler.
This is not an optical process. The robot uses capacitance sensors and a calibration probe (shipped with Flex pipettes) to perform automated calibration. Capacitive sensing for automated calibration is done by having a current run through the pipette’s metal tip at the bottom and then through a calibration tip until it’s grounded.
The Opentrons Flex 8-channel and 96-channel pipettes will aspirate and dispense the same volume with all channels at the same time. Volume is not independently controlled across channels.
OT-2 pipettes and OT-2 pipette tips are not compatible with the Opentrons Flex. The OT-2 pipette nose cone and Flex pipette nose cones are constructed differently, preventing cross-compatibility. OT-2 tip boxes/refills are also not compatible with the Flex.
Opentrons can only guarantee performance of the Flex when using our Flex pipette tips.