Small-scale protein purification for up to 96 samples using Biotage® PhyTip® columns
The OT-2 Dual Flow Chromatography Protein Purification Workstation includes OT-2 Robot, Pipettes, Tips, Labware and Custom Protocol Development
With the OT-2 Dual Flow Chromatography (DFC) Protein Purification Workstation, you can automate your small-scale protein purification and proteomics sample processing. This Opentrons Protein Purification Workstation uses Biotage PhyTip columns to perform dual flow chromatography-based protein purification.
The OT-2 DFC Protein Purification Workstation can support the following workflow steps:
Reagent plate preparation
Dual flow chromatography using PhyTip columns
The following Biotage PhyTip Columns are available for the OT-2:
This workflow can be used for the following applications:
Screening and optimization of purification conditions
Enzymatic and chemical lysis
Capture of macromolecular complexes
High-throughput protein purification for downstream applications
The OT-2 DFC Protein Purification Workstation includes all the hardware and consumables you need to perform automated protein purification (with the exclusion of the PhyTip columns, which must be purchased from Biotage). It comes with Opentrons’ protocol development service, which provides you with a customized protocol unique to your assay. You’ll also enjoy free access to the Opentrons Protocol Designer, Python API, and Protocol Library.
For FAQs about the OT-2 and other products, click here.
No additional modules are required to automate your Dual Flow Chromatography workflows with our DFC Protein Purification Workstation. Purification is carried out within Biotage PhyTips (sold separately).
The following PhyTip Columns are available for the OT-2:
CaptureSelect IgG-Fc Hu
Normal Phase 1
POROS CaptureSelect IgM
ProPlus & ProPlus LX(MabSelect Sure™)
For more information on these PhyTip Columns, please visit this page.
Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.
You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.
You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!
If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.
Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.
Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.
The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated
Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.
Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.