In Protocol Designer, the default well order is top to bottom, left to right, but you can customize it for each step, allowing different arrangements for source and destination labware. Read More

This article clarifies the distinction between warnings and errors in Protocol Designer. Read More

In Protocol Designer, the Transfer step allows you to move liquid between wells. You can specify which pipette to use, the transfer volume, source and destination labware and wells, tip replacement, and the motion path to use when performing the Transfer. Read More

In Protocol Designer, the touch tip setting removes droplets from the pipette tip by gently tapping it against the four sides of the well. Read More

In Protocol Designer, you can adjust the tip position for aspirating and dispensing liquids, including advanced settings with positions different from the default. Utilize batch edit mode for efficient editing across multiple steps. Read More

In Protocol Designer, Thermocycler Module steps allow you to change the state of the block and lid temperatures, open and close the lid, and program a Thermocycler profile. Read More

This error occurs when your protocol has no steps in it. Refer to the Building a Protocol: Steps section of our Protocol Designer support articles to learn more about making steps. Read More

In Protocol Designer, Temperature Module steps allow you to set the temperature, deactivate the module, and use the Pause step to create a gating temperature, pausing your protocol until that temperature condition is met. Read More

Protocol Designer only accepts JSON files created within the tool, and Opentrons cannot guarantee successful reuploading if the file is modified outside of Protocol Designer. Read More

In Protocol Designer, follow the method outlined in this article to create a serial dilution with a constant transfer volume in a single Transfer step. Read More