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Viscous Liquid Handling Automation using Opentrons OT-2

Molecular assays require precise pipetting reagents and samples with a diverse set of properties — including viscous ones that can clog tips, distribute unevenly and complicate reproducibility… Read More

Automation of bead-based ELISA forSARS-CoV-2 neutralizing antibody on the Opentrons OT-2 with an on-deck Heater-Shaker Module

Upon recognition of viral components, cell-mediated immune responses trigger secretion of neutralizing antibodies against viral antigens, for example nucleocapsid (N) protein and spike (S) protein present on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Read More

Executing Serial Dilutions On The OT-2

Serial dilutions are both independent experiments and building blocks of more complex experimental pipelines. Applications can be as simple as generating a standard curve or more complex phenotype characterization across cell culture concentrations. In any application, precise pipetting is required to generate reliable results. Read More

High performance automated nucleic acid extraction with the OT-2 platform

Modern molecular biology, driven by ever progressing technology, continues to deliver impressive and exciting new advancements — larger studies, more powerful therapeutics, and wholly new research questions. Read More

Sequence Ready Library Preparation Fully Automated For Microbial And Human DNA Template

The Swift 2S Turbo DNA Library Kit was fully automated using the Opentrons OT-2 for quick, hands-off, highquality libraries for Illumina Next-Generation Sequencing. Read More

Microbiome Nucleic Acid Extraction with ZymoBIOMICS Kit on OT-2

Microbiomics is a burgeoning field with implications for a wide range of topics in human health. Modern sequencing techniques can deliver rich and novel data but are constrained by DNA extraction methods that are difficult, time consuming, and, especially in microbiomics samples, prone to bias. Read More

Thesis: Robot-Trifle: A Robotic System for Automated Combinatorial Growth Phenotyping

A master’s thesis from Jeremy Kimball at the University of Illinois describing a streamlined, automated analysis pipeline to create microorganism growth phenotypes. Read More

Measuring Luteinising Hormone Pulsatility with a Robotic Aptamer-Enabled Electrochemical Reader

Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. … Read More

Fully Automated Sample Processing and Analysis Workflow for Low-Input Proteome Profiling

Recent advances in sample preparation and analysis have enabled direct profiling of protein expression in single mammalian cells and other trace samples. Several techniques to prepare and analyze low-input samples employ custom fluidics for nanoliter sample processing and manual sample injection onto a specialized separation column. … Read More

A Semiautomated Paramagnetic Bead-Based Platform For Isobaric Tag Sample Preparation

The development of streamlined and high-throughput sample processing workflows is important for capitalizing on emerging advances and innovations in mass spectrometry-based applications. While the adaptation of new technologies and improved methodologies is fast paced, automation of upstream sample processing often lags. Read More