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Automated RNA extraction using Zymo Research’s Direct-Zol RNA kit for samples stored in TRIzol®

In this study, the Opentrons OT-2 Nucleic Acid Extraction Workstation was used with the Zymo Research Direct-Zol 96 MagBead RNA kit to automate RNA extraction and purification. RNA quality, yield, and integrity were determined for 12 HeLa samples stored in TRIzol and an assessment to detect cross-contamination between plate wells… Read More

Thermocycler GEN2 White Paper

The Opentrons Thermocycler Module is a fully automation on-deck thermocycler, providing hands-free PCR in a 96-well plate format. It is compatible with all Opentrons robots and software to allow for fully automated PCR reaction setup and thermocycling on your Opentrons robot. Read More

Heater-Shaker Quickstart Guide

Heater-Shaker Module Instruction Manual

The Opentrons Heater-Shaker Module GEN1 provides on-deck heating and orbital shaking. The module can be heated to 95 °C, and can shake samples from 200 to 3000 rpm. Read More

Thermocycler Module Quickstart Guide

The Opentrons Thermocycler Module GEN2 provides ondeck, fully automated thermocycling. The module enablesautomation of upstream and downstream workflow steps… Read More

Thermocycler GEN2 Instruction Manual

Fully automated hybridization-based targeted sequencing sample prep with superior uniformity in 6 hours: A Point-n-Seq® workflow on Opentrons OT-2.

Automation has become increasingly vital to accommodate the growing demands of sample throughput, reliability, and reduced hands-on-time. Read More

Automated NGS Quantification, Normalization, and Pooling Using the OT-2

The Opentrons® OT-2 effectively performs qPCR quantification of NGS libraries, maintaining high precision while reducing hands-on time. Two methods of sample normalization were tested: individually for each sample or pooled with enzymatic normalization. Read More

Volatile Liquid Handling with Opentrons OT-2

Volatile liquids, liquids that evaporate readily at room temperature, are common reagents in various applications. Read More

Viscous Liquid Handling Automation using Opentrons OT-2

Molecular assays require precise pipetting reagents and samples with a diverse set of properties — including viscous ones that can clog tips, distribute unevenly and complicate reproducibility… Read More