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Opentrons Pipettes White Paper

Opentrons GEN2 Pipettes are designed specifically for lab automation and optimized for use with the OT-2. The Opentrons Single-Channel GEN2 pipettes reliably conduct liquid transfers from 1 µL -1,000 µl, while the Opentrons 8-Channel GEN2 pipettes reliably conduct liquid transfers from 1 µL -300 µl. Both have tip pickup and… Read More

Robust and high-throughput SARS-CoV-2 viral RNA detection, research, and sequencing using RNAdvance Viral and the OT-2 platform

Here we demonstrate an automated SARS-CoV-2 RNA extraction workflow using a Beckman Coulter Life Sciences RNAdvance Viral kit (C63510) on an Opentrons OT-2 robot; an open-source robotic liquid handling platform. Read More

Automated library preparation of small genomes using DNA preparation kits (Illumina DNA Prep Kit, KAPA HyperPrep kit, and NEBNext Ultra II Kit)

Three library prep kits – Illumina® DNA Prep Kit, KAPA® HyperPrep kit, and NEBNext® Ultra™ II Kit – were automated with the Opentrons OT-2 for fast and robust preparation of high quality libraries for next generation sequencing. Read More

Adapter Magnets Support Article

If the Magnetic Module is too weak for your application, this method can be used to strengthen its magnetic force to improve bead pelleting. Read More

Magnetic Module White Paper

The Opentrons Magnetic Module is a device that automatically engages and disengages high-strength neodymium magnets to seated well plates within the module housing. Read More

Automating Sensitive Polymerase Chain Reaction Protocols Using the Opentrons Thermocycler on the Opentrons OT-2 Platform

There is an increase in demand for sensitive polymerase chain reaction (PCR) techniques to be automated. In this application note, we demonstrate that the Opentrons Thermocycler and the OT-2 generate the expected base-pair length for PCR amplicons when compared to a popular off-deck third-party thermocycler. Read More

Swift 2S Turbo DNA Library Kit Protocol: Fully Automated

With this protocol, your OT-2 can fully automate the entire Swift 2S Turbo DNA Library Kit. Simply press start and your OT-2 can automate this entire workflow without any hands-on requirement – from enzymatic prep to sequence ready libraries! Up to 16 libraries can be prepared in under 3 hours. Read More

Temperature Module White Paper

The Opentrons Temperature Module is a hot and cold plate module that is able to maintain steady state temperatures for use within the OT-2 liquid handler or as a standalone benchtop product. Read More

Automated Protein Purification with Biotage Dual Flow Chromatography PhyTip Columns on the OT-2

An automated protocol was developed to perform dual flow chromatography using Biotage® PhyTip® columns on the Opentrons OT-2 liquid handler. Read More

Automated Ni-NTA bead-based protein purification on the OT-2 liquid handler

An automated protocol was developed to perform magnetic bead-based immobilized metal affinity chromatography (IMAC) on the Opentrons OT-2 liquidhandler. Read More