The Opentrons OT-2 Liquid Handler User Manual contains important information regarding the safe use of the model OT-2R Liquid Handler. Read and understand the safety information thoroughly before operating the Opentrons Liquid Handler. Read More

Opentrons GEN2 Pipettes are designed specifically for lab automation and optimized for use with the OT-2. The Opentrons Single-Channel GEN2 pipettes reliably conduct liquid transfers from 1 µL -1,000 µl, while the Opentrons 8-Channel GEN2 pipettes reliably conduct liquid transfers from 1 µL -300 µl. Both have tip pickup and… Read More

Here we demonstrate an automated SARS-CoV-2 RNA extraction workflow using a Beckman Coulter Life Sciences RNAdvance Viral kit (C63510) on an Opentrons OT-2 robot; an open-source robotic liquid handling platform. Read More

Three library prep kits – Illumina® DNA Prep Kit, KAPA® HyperPrep kit, and NEBNext® Ultra™ II Kit – were automated with the Opentrons OT-2 for fast and robust preparation of high quality libraries for next generation sequencing. Read More

If the Magnetic Module is too weak for your application, this method can be used to strengthen its magnetic force to improve bead pelleting. Read More

The Opentrons Magnetic Module is a device that automatically engages and disengages high-strength neodymium magnets to seated well plates within the module housing. Read More

There is an increase in demand for sensitive polymerase chain reaction (PCR) techniques to be automated. In this application note, we demonstrate that the Opentrons Thermocycler and the OT-2 generate the expected base-pair length for PCR amplicons when compared to a popular off-deck third-party thermocycler. Read More

With this protocol, your OT-2 can fully automate the entire Swift 2S Turbo DNA Library Kit. Simply press start and your OT-2 can automate this entire workflow without any hands-on requirement – from enzymatic prep to sequence ready libraries! Up to 16 libraries can be prepared in under 3 hours. Read More

The Opentrons Temperature Module is a hot and cold plate module that is able to maintain steady state temperatures for use within the OT-2 liquid handler or as a standalone benchtop product. Read More

An automated protocol was developed to perform dual flow chromatography using Biotage® PhyTip® columns on the Opentrons OT-2 liquid handler. Read More