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Leveling 8-Channel pipettes

8-Channel pipettes are especially sensitive to tilt and must be leveled flat during the pipette attachment process. An improperly attached or unleveled 8-Channel pipette can affect the reliability of tip pickup and ejection, evenness of aspiration and dispensation, and accuracy of calibration. Read More

How to adjust the aspirate or dispense height

This article covers how to adjust the height at which pipettes aspirate and dispense in both Python API and Protocol Designer protocols. Read More

GEN2 pipette compatibility

This article outlines compatibility considerations between GEN1 and GEN2 pipettes for use with OT-One and OT-2 robots. Read More

Fix tip probe switch collisions caused by a bad factory calibration

Normally, when you calibrate your pipettes, each pipette automatically taps five tip probe switches hidden under the OT-2’s trash bin. The pipette lowers a safe distance from each switch, then slowly moves towards the switch until it taps it. On some OT-2s, the pipette can lower… Read More

Finding your pipette’s serial number

Every pipette has a unique serial number located on the back of the physical instrument and in the Opentrons App if it’s currently attached. Read More

Do I have a GEN1 pipette or a GEN2 pipette?

Refer to the information below to determine the generation of your pipette for use with the OT-One or OT-2 robot. Read More

Detach a pipette

This article provides step-by-step instructions on detaching a pipette from your robot within the Opentrons App. Read More

Chemical resistance of Opentrons pipettes

Opentrons pipettes are designed and tested to handle a wide range of aqueous solutions common to biochemistry. Read More

Checkit Go pipette testing system

Checkit Go is an innovative testing system specifically designed to quantitatively assess the accuracy of multi-channel pipettes on liquid handling robots. By effectively measuring the accuracy of various pipette models, this system helps identify any potential volume delivery errors. Read More

Changing advanced pipette settings

This article is a resource to supplement working with the Opentrons Support team to troubleshoot pipette issues (e.g. pipette tips not picking up, pipette tips not dropping), in addition to instructions on how to tune pipette parameters in the Opentrons App without having to make adjustments to a protocol. Read More