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Knowledge Hub

Support Articles

Recalibrating tip length before running a protocol

This article provides instructions on recalibrating your pipette’s tip length and pipette offset before running a protocol in the event…

Support Articles

How positional calibration works on the OT-2

The OT-2 moves pipettes around in 3D space. To do that accurately and precisely, it needs to be calibrated. This article…

Support Articles

How Labware Offsets work on the OT-2

This article describes how Labware Offset data is utilized during the Labware Position Check workflow when running a protocol on…

Support Articles

Check your OT-2’s calibration health

Running a Calibration Health Check through the Opentrons App can help ensure your OT-2’s positional calibration…

Support Articles

Write and simulate protocols on Windows 10

There are quite a few ways to write and simulate Opentrons Python protocols on your computer, but installing and using…

Support Articles

What are dead volumes, and how do I reduce them?

Dead volume refers to the residual volume of liquid left in the well or lost to waste that cannot be…

Support Articles

Using modules of the same type on the OT-2

This article provides detailed guidance on incorporating multiple modules of the same type within Python API protocols, which enable the…

Support Articles

Temperature and humidity recommendations for the OT-2

This article outlines the recommended temperature and humidity conditions for optimal performance of the OT-2.

Support Articles

Table recommendations for the OT-2

This article offers guidance on the recommended table specifications for the OT-2 to ensure smooth operation and optimal performance.

Support Articles

Refilling a tip rack

Opentrons tip racks feature a separate base and detachable refill rack. Refer to the illustrations below to…

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