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Export your protocol

While using Protocol Designer, Opentrons recommends exporting your file frequently because the browser will not save your work.

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During a run, pipette tips don’t go to the bottom of labware

If you encounter this issue in a Protocol Designer protocol, follow the steps outlined in this article to correct the…

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Delay

In Protocol Designer, you can add a delay to both aspirate and dispense actions. A delay holds the tip at…

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Continuity errors

In Protocol Designer, continuity errors can happen when you edit liquids, labware, or steps upstream.

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Change tip

In Protocol Designer, you can instruct the robot on when to acquire a new tip in any step involving a…

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Batch editing steps

In Protocol Designer, batch edit mode enables you to edit multiple Transfer or Mix steps simultaneously, streamlining your protocol editing…

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Air gap

In Protocol Designer, air gap allows you to aspirate air into the tip after aspirating liquid from a source well.

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Advanced settings

In Protocol Designer, both Mix and Transfer pipetting steps have advanced settings. Use the gear across from Aspirate or Dispense…

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About the Protocol Designer

Protocol Designer enables the creation of simple pipetting protocols for Opentrons robots without the need for coding.

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A well has an unknown liquid in it

In Protocol Designer, if a well contains an unnamed or “unknown” liquid, it indicates that the pipette has aspirated more…

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