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Knowledge Hub

Support Articles

Warnings vs. errors

This article clarifies the distinction between warnings and errors in Protocol Designer.

Support Articles

Transfer

In Protocol Designer, the Transfer step allows you to move liquid between wells. You can specify which pipette to use,…

Support Articles

Touch tip

In Protocol Designer, the touch tip setting removes droplets from the pipette tip by gently tapping it against the four…

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Tip position

In Protocol Designer, you can adjust the tip position for aspirating and dispensing liquids, including advanced settings with positions different…

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Thermocycler Module steps

In Protocol Designer, Thermocycler Module steps allow you to change the state of the block and lid temperatures, open and…

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There are pipettes or no labware to calibrate when I try to run a protocol

This error occurs when your protocol has no steps in it. Refer to the Building a Protocol:…

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Temperature Module steps

In Protocol Designer, Temperature Module steps allow you to set the temperature, deactivate the module, and use the Pause step…

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Supported file types

Protocol Designer only accepts JSON files created within the tool, and Opentrons cannot guarantee successful reuploading if the file is…

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Quick method to build a serial dilution

In Protocol Designer, follow the method outlined in this article to create a serial dilution with a constant transfer volume…

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Pre-wet tip

In Protocol Designer, the pre-wet tip setting allows you to aspirate and dispense once in the source well before aspirating…

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