Protein purification can be complex and time-consuming. Automating your protein purification workflow can increase efficiency, reduce errors and save hands-on time. Protein purification methods that are most adaptable to automation use magnetic beads or dual flow chromatography columns.
The Opentrons Flex Absorbance Plate Reader Module is a fully automated solution for absorbance-based assays. Using the example of protein quantification by BCA assay, our scientists will show how to use an Opentrons Flex with the newly released on-deck absorbance plate reader to set up and run assays, and then normalize concentrations, all from a single protocol without human intervention.
Learn how to automate magnetic bead-based protein purification with the OT-2 using Thermo Fisher Dynabeads™ Protein A/Protein G & Thermo Fisher Pierce™ Ni-NTA Magnetic Agarose Beads.
Automate protein purification on the Opentrons Flex
With the Flex Magnetic Bead Protein Purification Workstation, you can automate your bead-based protein purification workflow at the scale you need to increase efficiency, reduce errors, and save hands-on time. Flex enables you to automate small-scale isolation of specific proteins, protein complexes, and antibodies, or proteomics sample processing, using many leading magnetic bead-based reagent systems on the market.
Use the OT-2 to automate small-scale, dual flow chromatography protein purification using Biotage® PhyTip® columns.
Perform dual flow chromatography using Biotage® PhyTip® columns on the Opentrons OT-2 liquid handler.
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*Verification categories:
Opentrons = has been verified by the Opentrons Team, who verify that the scientific results are as expected.
Partner = has been verified by our reagent partner, who verifies that the scientific results are as expected.
Community = protocol is in use by members of the Opentrons community.
Our team of experts can help figure out if automation is right for you. Book a virtual demo to discuss your workflow needs with an expert.
