Automation of Luminescence Immunoassay Sample Prep 

Automating LIA sample prep involves using specialized technologies and systems to streamline the process:

• • Sample Loading: Samples (cells, tissues, or fluids) are automatically loaded into processing wells or tubes by robotic arms or liquid handlers. This step ensures precise and contamination-free handling.
  • Reagent Dispensing:Add capture antibodies, buffers, and other necessary reagents using robotic liquid handlers.
  • Incubation: Maintain controlled temperature and timing for optimal binding of analytes to capture molecules.
  • Washing:Use automated plate washers to remove unbound substances and reduce background noise.
  • Detection Reagent Addition: Dispense luminescent substrates or labeled detection antibodies.
  • Final Incubation: Allow sufficient time for signal development through enzymatic or chemical reactions.
  • Signal Detection: Transfer plates to a luminescence plate reader for measurement and data capture.

Automate LIA Sample Prep with Opentrons:

Interested in automating the reagent dispensing steps in your lab’s luminescence immunoassay Sample Prep workflows? Enter the Opentrons Flex, a walk up, no-code pipetting robot that scales with your lab. Let us handle the pipetting while you spend more time on science.

Benefits of Automation Over Manual Methods

• Increased Speed:Automated systems significantly speed up the sample preparation process, allowing for higher throughput and faster turnaround times, which is especially valuable in large-scale studies.
• Improved Reproducibility: Automation ensures consistent sample preparation by minimizing human error and variability between different samples or operators, leading to more reliable results.
• Higher Throughput: Automation allows for handling larger sample volumes simultaneously, ideal for high-throughput projects.
• Better Accuracy and Precision: Robots perform tasks with higher precision, reducing contamination and errors.
• Cost Savings: Though the upfront cost can be higher than manual methods, automation reduces labor and reagent waste in the long run.

Methods of Luminescence Immunoassay Sample Prep

Magnetic Bead-Based Separation 

Uses antibody-coated magnetic beads to capture target analytes, allowing for easy washing and separation with a magnet.

Microplate-Based Assays

Involves immobilizing capture antibodies on plate wells for high-throughput processing and direct luminescence detection.

Centrifugal Filtration

Employs membrane filters and centrifugation to concentrate and purify proteins or other analytes.

Precipitation Methods

Uses chemical agents to selectively precipitate proteins or other interfering substances before analysis.