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Saving and uploading

While using Protocol Designer, export your file frequently as the browser does not automatically save your work. Any JSON file created in Protocol Designer can be imported and edited. Read More

What you need to use the Protocol Designer

Protocol Designer must be accessed through the web browser Google Chrome and requires an active internet connection. Read More

Well order

In Protocol Designer, the default well order is top to bottom, left to right, but you can customize it for each step, allowing different arrangements for source and destination labware. Read More

Warnings vs. errors

This article clarifies the distinction between warnings and errors in Protocol Designer. Read More

Transfer

In Protocol Designer, the Transfer step allows you to move liquid between wells. You can specify which pipette to use, the transfer volume, source and destination labware and wells, tip replacement, and the motion path to use when performing the Transfer. Read More

Touch tip

In Protocol Designer, the touch tip setting removes droplets from the pipette tip by gently tapping it against the four sides of the well. Read More

Tip racks

In Protocol Designer, refer to the guidelines in this article to streamline your tip rack setup and management for efficient pipetting. Read More

Tip position

In Protocol Designer, you can adjust the tip position for aspirating and dispensing liquids, including advanced settings with positions different from the default. Utilize batch edit mode for efficient editing across multiple steps. Read More

Thermocycler Module steps

In Protocol Designer, Thermocycler Module steps allow you to change the state of the block and lid temperatures, open and close the lid, and program a Thermocycler profile. Read More

There are pipettes or no labware to calibrate when I try to run a protocol

This error occurs when your protocol has no steps in it. Refer to the Building a Protocol: Steps section of our Protocol Designer support articles to learn more about making steps. Read More