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Support Articles

Continuity errors

In Protocol Designer, continuity errors can happen when you edit liquids, labware, or steps upstream.
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Change tip

In Protocol Designer, you can instruct the robot on when to acquire a new tip in any step involving a…
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Batch editing steps

In Protocol Designer, batch edit mode enables you to edit multiple Transfer or Mix steps simultaneously, streamlining your protocol editing…
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Air gap

In Protocol Designer, air gap allows you to aspirate air into the tip after aspirating liquid from a source well.
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Advanced settings

In Protocol Designer, both Mix and Transfer pipetting steps have advanced settings. Use the gear across from Aspirate or Dispense…
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About the Protocol Designer

Protocol Designer enables the creation of simple pipetting protocols for Opentrons robots without the need for coding.
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A well has an unknown liquid in it

In Protocol Designer, if a well contains an unnamed or “unknown” liquid, it indicates that the pipette has aspirated more…
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How to handle viscous liquids in Protocol Designer

This article offers practical recommendations for effectively managing viscous liquids within Protocol Designer protocols, focusing on optimizing liquid handling techniques.
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With two pipettes attached, one is slightly off-position during protocol runs

This is a known limitation in software versions earlier than 4.0.0. During a protocol run, when the pipettes move to…
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Why is my pipette going straight to the bottom of my labware when it is full of liquid?

The OT-2 doesn’t have embedded motors so it defaults to the bottom of the labware it is aspirating and dispensing…
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