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ot2 tech specs
Dimensions

63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H)
Takes up half of your standard lab bench

SPECSOT2

Weight

48 kg or 105.8 lb

Pipette configurations

Single- and 8-channel pipetting
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.

Pipette volumes and specs

Single-channel: 1-1000 µL
8-channel: 1-300 µL

Deck

11 deck slots enable countless configurations
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.

Connectivity

WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0

Video

Integrated video camera (Included for future or custom functionality)

Audio

Integrated audio system (Included for future or custom functionality)

Power requirements

Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information, please review this support article

Frame composition

Rigid steel and CNC aluminum design

Operating temperature & relative humidity

Recommended 20-24 °C
Recommended 40-60% RH

Time to fill 96-well plate
Pipette Distribute,
one tip
Transfer,
same tip
Transfer,
new tip
P20 8-Channel 0:22 1:35 4:19
P300 8-Channel 0:26 1:38 4:24
P20 Single-Channel 3:12 13:10 36:01
P300 Single-Channel 4:01 13:39 41:31
P1000 5:54 15:21 44:50
Single-channel accuracy & precision
Pipette Volume Random Error Systematic Error
%D µl %CV µl
P20 1
10
20
±15%
±2%
±1.5%
0.15µl
0.2µl
0.3µl
±5%
±1%
±0.8%
0.05µl
0.1µl
0.16µl
P300 20
150
300
±4%
±1%
±0.6%
0.8µl
1.5µl
1.8µl
±2.5%
±0.4%
±0.3%
0.05µl
0.6µl
0.9µl
P1000 100
500
1000
±2%
±1%
±0.7%
2.0µl
5.0µl
7.0µl
±1%
±0.2%
±0.15%
1µl
1µl
1.5µl
8-channel accuracy & precision
Pipette Volume Random Error Systematic Error
%D µl %CV µl
P20 1
10
20
±20%
±3%
±2.2%
0.2µl
0.3µl
0.44µl
±10%
±2%
±1.5%
0.1µl
0.2µl
0.3µl
P300 20
150
300
±10%
±2.5%
±1.5%
2.0µl
3.75µl
4.5µl
±4%
±0.8%
±0.5%
0.8µl
1.2µl
1.5µl
Sterility

Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights

Viscosity
For best practices on automating viscous liquid handling, review our application note
API compatibility

Interfaces with any product that has an API
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.

Interface
Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later.
Tip compatibility

Use Opentrons Tips and Opentrons Filter Tips
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.

Labware compatibility

Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.

Warranty

One year warranty against manufacturer defects
Stress tested to last a minimum of 3 years of full-time use

Return policy
60 day, 100% risk free
Certifications

Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)

Example Commands

Tip Handling

  • Pick up tip
  • Drop tip
  • Return tip
  • New tip (always, never, once)
  • More…

Liquid Control
  • Aspirate
  • Dispense
  • Aspirate speed
  • Dispense speed
  • Blowout
  • Touch tip
  • Mix
  • Air gap

Utility

  • Move to
  • Delay
  • User-specified pause
  • Homing
  • Comment


Transfer

  • Basic transfer
  • Volumes > Pipette Volume
  • Distribute
  • Consolidate


Variable Settings

  • Speed
  • Volume
  • Count


Other

  • Dispose
What’s in the box

BOX

OT-2 robot
Calibration block
Removable block
Trash container
Quickstart guide
Power supply and regional IEC cable
Ethernet cable
Ethernet-to-USB dongle
Tool kit