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What’s New – Opentrons App 9.0

Protocol Visualization

Protocol Visualization brings your workflows to life, allowing you to view and simulate protocols before execution, whether they’re created through Opentrons AI, Protocol Designer, or the Python API.

20 µL Tips + API Support

With the launch of 20 µL tips and full API support, Opentrons enables high-accuracy low-volume liquid handling for sensitive workflows like NGS prep, PCR setup, and assay miniaturization.

Miscellaneous Improvements

Quick Transfer updates: integrated into the Protocols tab, with pinning and run tracking like standard protocols-



Liquid class enhancements (API 2.28): improved blowout control, efficiency fixes, and expanded configurability



Partial tip pickup + return tip support for more flexible tip usage



Protocol visualization improvements: clearer differentiation of aspirate, dispense, and air gap steps



System-level updates: ongoing improvements to robot performance and execution reliability

For a full list of changes on all Opentrons App versions, please visit our GitHub below. Please contact our Support team at [email protected] with any questions or concerns