Opentrons logo
Cancel

Frequently Asked Questions

For FAQs about the OT-2 and other products, click here.

Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.

No additional modules are required to automate your Dual Flow Chromatography workflows with our DFC Protein Purification Workstation. Purification is carried out within Biotage PhyTips (sold separately).

You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.

Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.

Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.

The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated

You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!

If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.

Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.

The following PhyTip Columns are available for the OT-2:

C18 300Ã…
C18 90Ã…
C4 300Ã…
CaptureSelect IgG-Fc Hu
CaptureSelect IgG-Fc
CaptureSelect KappaXL
CaptureSelect IgG-Fc-ms
Glutathione
IMAC
Normal Phase 1
POROS CaptureSelect IgM
ProPlus & ProPlus LX(MabSelect Sureâ„¢)
Protein A
Protein G
Streptavidin
Strong Anion
Strong Cation
Weak Anion

For more information on these PhyTip Columns, please visit this page.