Automating NGS Library Prep

Automated workflows designed with flexibility for high-throughput labs—eliminate bottlenecks, reduce cost per sample, and run at full capacity.

Access application support and and a library of protocols

As sample volumes grow, manual and semi-automated library prep quickly become limiting factors. More samples shouldn’t require more time, or more risk. Reference the Opentrons Protocol Library as templates of NGS protocols to reduce optimization time and increase the efficiency of library construction. Contact our Field Application Scientists to adapt existing workflows to the needs of your team.

Protocol Library

Flex NGS Workstation

Optimize your throughput with the Flex NGS Workstation—a complete solution for automating your workflow, enabling you to accelerate your scientific discoveries. The Flex NGS Workstation allows for scalable workflow automation to enhance efficiency, minimize errors, and conserve hands-on time.

What’s Included

Discover the Opentrons Flex High Throughput NGS Workstation

Hear how your lab can move from throughput limitations to fully optimized, high-volume processing directly from our customers.

Watch the Webinar

“

The Flex very materially helps us do library prep and saves time and mental energy. More importantly it changes how we think about things. How can we systematize this so it can be done more efficiently overall? I like that culture overall. It’s a much more high-performing way to do things.

– Peter McCaffrey, MD, FCAP, Assistant Professor: UTMB, CTO: Pragma Bio

Resources

Reagent	Application Data	Protocols	Verification *
QIAGEN QIAseq FX DNA Library Kit	Automating DNA Library Prep QIAseq FX plus Normalizer on Opentrons Flex	QIAseq FX DNA Library Kit on Opentrons Flex QIAseq FX DNA Library Kit with QIAseq Normalizer Workflow B on Opentrons Flex	Opentrons Partner
QIAGEN QIAseq Library Normalizer Kit	Automating DNA Library Prep QIAseq FX plus Normalizer on Opentrons Flex	QIAseq Library Normalizer Kit Workflow A on Opentrons Flex	Opentrons Partner
Illumina DNA Prep Kit	Automated library preparation of small genomes using DNA preparation kits on OT-2	Illumina DNA Prep on Opentrons Flex (coming soon) Illumina DNA Prep on OT-2	Opentrons
IDT xGEN DNA EZ and MC Library Prep Kits		IDT xGEN DNA EZ Library Prep Kit on Opentrons Flex IDT xGEN DNA EZ Library Prep Kit on OT-2 IDT xGEN DNA MC Library Prep Kit on OT-2	Opentrons
IDT xGEN Normalase Module		NGS Library Normalization with IDT xGEN Normalase Module on OT-2	Opentrons
seqWell ExpressPlex Library Prep Kit	[Poster] Automated, Multiplexed Preparation of up to 384 Plasmid Libraries Supports a 24-Hour End-to-End Sequencing Workflow	seqWell ExpressPlex Library Preparation on Opentrons Flex (2-part protocol)	Opentrons Partner
Roche KAPA HyperPrep Kit	Automated library preparation of small genomes using DNA preparation kits on OT-2	Roche KAPA HyperPrep Kit on Opentrons Flex (coming soon) Roche KAPA HyperPrep Kit on OT-2	Opentrons
NEBNext Ultra II DNA Library Prep Kit for Illumina	Automated library preparation of small genomes using DNA preparation kits on OT-2	NEBNext Ultra II DNA Library Prep Kit for Illumina on OT-2	Opentrons Partner
Nextera XT DNA Library Preparation Kit		Illumina Nextera XT on OT-2 (4-part protocol)	Community

*Verification categories:
Opentrons = has been verified by the Opentrons Team, who verify that the scientific results are as expected.
Partner = has been verified by our reagent partner, who verifies that the scientific results are as expected.
Community = protocol is in use by members of the Opentrons community.

Want to know more?

Our team of experts can help figure out if automation is right for you. Book a virtual demo to discuss your workflow needs with an expert.

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