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NGS Workstation e1678723883759
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OT-2 NGS Workstation


End-to-end NGS library prep of up to 24 samples

The NGS Workstation includes OT-2 Robot, Pipettes, Tips, Labware, Thermocycler, Magnetic and Temperature Modules, and Custom Protocol Development.

Automating NGS Library Prep

With the OT-2 NGS Workstation, you can automate your library prep and pre-sequencing workflows using any leading reagent system on the market, including fragmentation and tagmentation protocols.


Library Construction


Bead Clean Ups


Normalization and Pooling


Library & Sample Prep

Well Plates

Tagmentation & Transformation

What’s in the box

Each OT-2 NGS Workstation is configured with hardware, consumables, and custom protocol development for your NGS assay.

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Robot & Pipettes

  • OT-2
  • 8-Channel GEN2 Pipette (P20)
  • 8-Channel GEN2 Pipette (P300)
  • Custom Protocol Development
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  • Magnetic Module
  • Temperature Module
  • Thermocycler Module
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  • 2 Cases Opentrons 20 uL Filter Tip Racks (2 x 9,600 tips)
  • 2 Cases Opentrons 200 uL Filter Tip Racks (2 x 9,600 tips)
  • NEST 12-Well Reservoirs (50 count)
  • Tough 0.2 mL 96-Well PCR Plates (25 count)
  • NEST 2.0 mL Microcentrifuge Tubes (500 count)

Demonstrated Method for DNA preparation on the OT-2 NGS Workstation

Demonstrated Method for DNA preparation on the OT-2 NGS Workstation

ngs ot2 figure
Figure 1. NGS with OT-2. The dotted line box indicate the NGS steps that can be automated on the OT-2

Frequently Asked Questions

For FAQs about the OT-2 and other products, click here.

We’ve validated protocols for:

Illumina® DNA Prep Kit

KAPA® HyperPrep kit

NEBNext® Ultra™ II Kit

Omega Bio-Tek’s Mag-Bind TotalPure NGS

RNAdvance Viral

You can browse all of our community’s NGS Library Prep protocols within our Protocol Library. Don’t see a protocol for the kit your lab is using? We offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.

Our NGS Workstation can fully automate library preparation for up to 24 samples per run. Increased throughputs are possible, however manual steps (e.g. swapping out tip racks) would be required.

One of our Automation Specialists would be happy to walk through the set-up that would work best for your lab’s throughput requirements via a quick consultation.

Out of the box, the OT-2 can support any automation-compatible, ANSI/SLAS compliant well plates, plus a wide range of snap-cap and screw-cap tubes up to a 50 mL conical with our 4-in-1 Tube Rack. You can see the full range of supported labware in our Labware Library.

You do not need to know how to code to use the Opentrons platform. Protocols can be created using Opentrons’ plug-and-play protocol design tools. We also have an Applications Engineering team that can build custom protocols for you.

You can find our community’s collection of OT-2 protocols within our Protocol Library. Feel free to search the library for a protocol that matches your lab’s workflows!

If you don’t see a protocol that matches your lab’s processes, we offer a couple different options for creating your protocol. Additionally, you can leverage our team of engineers for a custom protocol development.

Due to the open-source nature of the robot, many of our users have found success integrating the OT-2 with third party lab equipment.

Integrations such as these would be a DIY project taken on by your lab, and would not fall under the scope of the Opentrons Support team.

The OT-2 has the following certifications: CE, FCC, NRTL, ISO 9001, CB
Note that the robot is not IVD or GMP certified/validated

Labs within the Opentrons community have installed the OT-2 pipetting robot in biological safety cabinets (as well as in inert atmosphere glove boxes), including Labconco’s Logic Vue Class II enclosures and Baker’s AeroPROTECT 360 enclosures. We recommend you cross-check the dimensions of the OT-2 with those of your chosen biological safety cabinet.

Risk free 60-day demo

Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.