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How to handle viscous liquids in Protocol Designer

This article offers practical recommendations for effectively managing viscous liquids within Protocol Designer protocols, focusing on optimizing liquid handling techniques. Read More

With two pipettes attached, one is slightly off-position during protocol runs

This is a known limitation in software versions earlier than 4.0.0. During a protocol run, when the pipettes move to labware, you might notice one pipette goes to the correct place and the other pipette is up to 1-2 mm off, and that error is consistent throughout the protocol. Read More

Why is my pipette going straight to the bottom of my labware when it is full of liquid?

The OT-2 doesn’t have embedded motors so it defaults to the bottom of the labware it is aspirating and dispensing from if you don’t tell it to do otherwise. This article reviews how to change the aspiration/dispense height in the Python API and Protocol Designer if you are aspirating from… Read More

Why is my 8-Channel pipette crashing into my Opentrons module?

When creating a protocol using 8-Channel pipettes and modules, there are certain deck layouts that will cause the pipette to crash into your modules. To prevent these crashes, you will want to avoid placing labware (including tip racks) requiring 8-Channel access directly adjacent to your modules. Read More

Troubleshooting Windows blue screen errors

Blue screen (BSOD) errors on Windows have been rarely reported with the OT-2, but you may encounter them during protocol uploads or software updates. Read More

Troubleshooting USB connection problems on Windows

This article provides guidance on verifying the proper configuration of the network interface to ensure your computer can access the OT-2 through a wired connection. Read More

Troubleshooting USB connection problems on Ubuntu

This article provides guidance on verifying the proper configuration of the network interface to ensure your computer can access the OT-2 through a wired connection. Read More

Troubleshooting USB connection problems on macOS

This article provides guidance on verifying the proper configuration of the network interface to ensure your computer can access the OT-2 through a wired connection. Read More

Troubleshooting tip pickup problems

These troubleshooting steps address issues such as tips unexpectedly dropping, pipette not aspirating properly, and uneven aspiration across channels of a multi-channel pipette. Read More

Troubleshooting problems with Wi-Fi connections

If you’re unable to establish a Wi-Fi connection to the OT-2, follow the steps outlined in this article. Read More