In Protocol Designer, Temperature Module steps allow you to set the temperature, deactivate the module, and use the Pause step to create a gating temperature, pausing your protocol until that temperature condition is met. Read More

Protocol Designer only accepts JSON files created within the tool, and Opentrons cannot guarantee successful reuploading if the file is modified outside of Protocol Designer. Read More

In Protocol Designer, follow the method outlined in this article to create a serial dilution with a constant transfer volume in a single Transfer step. Read More

In Protocol Designer, the pre-wet tip setting allows you to aspirate and dispense once in the source well before aspirating the Transfer volume. Read More

In Protocol Designer, insert a Pause step to halt the robot during a run. Specify whether it should automatically resume after a set time or wait for manual user input to continue. Read More

In Protocol Designer, there are several options, or paths, for how to control the robot’s movement between wells, including single transfer, multi-aspirate, and multi-dispense. Read More

In Protocol Designer, the mix setting performs a mixing action either before an aspirate or after a dispense within a Transfer step. Read More

In Protocol Designer, the Mix step involves the robot pipetting up and down within a well without transferring liquid to the next well. Read More

In Protocol Designer, you have the ability to name, duplicate, reorder, and delete steps for enhanced protocol customization. Read More

In Protocol Designer, Magnetic Module steps enable you to toggle between engaged and disengaged states as you build your protocol. Read More