In Protocol Designer, specifying the liquids in your protocol enables you to monitor well contents at every step and receive alerts when aspirating from an empty well. Read More

In Protocol Designer, labware compatibility is categorized into three tiers: Recommended, Compatible, and Incompatible. Read More

In Protocol Designer, you can interact with labware by nicknaming, moving, or deleting it. Note that it’s not possible to swap labware to a different type after placement. Read More

In Protocol Designer, if wells are unclickable or don’t appear when labware is selected, verify that previous steps are not empty and delete any that are. Read More

In Protocol Designer, switch pipettes between the left and right mount by selecting “Swap” on the File tab. Read More

If you encounter a bug while using Protocol Designer, this article provides instructions on how to report it. Read More

In Protocol Designer, you can assign different colors to each liquid in your protocol. This color-coding is visible on the deck map in both Protocol Designer and the Opentrons App. Read More

Using a Heater-Shaker with Protocol Designer is similar to other modules, but be mindful of specific limitations regarding module placement on the deck while designing your protocol. Read More

In Protocol Designer, adjusting the flow rate allows you to control the speed at which the robot aspirates or dispenses liquids. This is especially beneficial when working with viscous liquids, which may require a lower flow rate for optimal pipetting. Read More

In Protocol Designer, the last step in your protocol is the final deck state. By selecting this step, you can hover over your deck’s labware to view well contents and hover over wells to see the liquids inside. Read More