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Troubleshooting pipette connection problems

If your OT-2 is having difficulty communicating with the attached pipette, ensure the white connector tab is securely plugged into the pipette and recheck the connection. Read More

Troubleshooting USB connection problems

If your OT-2 isn’t appearing in the Opentrons App despite being connected via USB, use this guide to troubleshoot common connection issues. Read More

Troubleshooting calibration issues

There are two types of calibration-related technical issues: those hindering calibration processes and those affecting the robot’s movement during a protocol run. Read More

Troubleshoot calibration problems when the usual methods won’t help

Opentrons Support might ask you to follow these specific instructions if you’re unable to update your OT-2 to the latest software version to benefit from the newest calibration features and bug fixes or if the usual troubleshooting methods haven’t helped. Read More

The app says my protocol has no steps

Outdated OT-2 robot software may inaccurately identify some protocols as empty. Update both your Opentrons App and robot software to the latest version to resolve this issue. Read More

SmoothieError: What is it and how do I fix it?

A SmoothieError is how the OT-2 reports something that went wrong with a motor controller command. It is usually a symptom of an underlying mechanical or electrical problem. Read More

SmoothieError: Homing fail

A homing fail occurs when the limit switch was unexpectedly not hit while the OT-2 was homing. Homing fails usually happen if a) you’re using different hardware with a different axis length or b) the axis is stuck or skipping during the home. Read More

SmoothieError: Hard limit

A hard limit occurs when a limit switch was unexpectedly hit while the OT-2 was not homing. Essentially, it is when an endstop triggers when it is not supposed to (e.g. outside of a home). The error message will mention the axis that failed, like “hard limit +X.”… Read More

Retrieving system information

This article describes how to obtain the information required to troubleshoot connection issues on a Windows 10 machine. Read More

Resolving a “Bad deck calibration data detected! This robot is likely to experience a crash” error

Encountering this error on your OT-2 prevents protocol uploads and runs. Resolve it by updating the Opentrons App and OT-2 robot software to the latest version and calibrating the deck. Read More