While using Protocol Designer, Opentrons recommends exporting your file frequently because the browser will not save your work. Read More

If you encounter this issue in a Protocol Designer protocol, follow the steps outlined in this article to correct the tip position. Read More

In Protocol Designer, you can add a delay to both aspirate and dispense actions. A delay holds the tip at a position for a defined amount of time after aspirating or dispensing a liquid. Read More

In Protocol Designer, continuity errors can happen when you edit liquids, labware, or steps upstream. Read More

In Protocol Designer, you can instruct the robot on when to acquire a new tip in any step involving a pipette. Note that the available choices are limited based on the type of Transfer you are configuring. Read More

In Protocol Designer, batch edit mode enables you to edit multiple Transfer or Mix steps simultaneously, streamlining your protocol editing process. Read More

In Protocol Designer, air gap allows you to aspirate air into the tip after aspirating liquid from a source well. It’s typically used to prevent liquid from dripping out of the tip. Air gap can look a little different depending on the Transfer path you have selected. Read More

In Protocol Designer, both Mix and Transfer pipetting steps have advanced settings. Use the gear across from Aspirate or Dispense headers to access these settings. Read More

Protocol Designer enables the creation of simple pipetting protocols for Opentrons robots without the need for coding. Read More

In Protocol Designer, if a well contains an unnamed or “unknown” liquid, it indicates that the pipette has aspirated more than the volume of a source well. Read More