Automated Protein Quantification Assays for the Opentrons Flex

This app note describes the automation of a restriction enzyme-free and ligation-free cloning workflow using the In-Fusion Snap Assemblyon the OT-2… Read More

Mass Balance Integration with the OT-2

By integrating a mass balance with the Opentrons OT-2 Robot, users can automate gravimetric experiments and accurately record how much liquid mass is added to a sample over time. Read More

Automated RNA extraction using Zymo Research’s Direct-Zol RNA kit for samples stored in TRIzol®

In this study, the Opentrons OT-2 Nucleic Acid Extraction Workstation was used with the Zymo Research Direct-Zol 96 MagBead RNA kit to automate RNA extraction and purification. RNA quality, yield, and integrity were determined for 12 HeLa samples stored in TRIzol and an assessment to detect cross-contamination between plate wells… Read More

Fully automated hybridization-based targeted sequencing sample prep with superior uniformity in 6 hours: A Point-n-Seq® workflow on Opentrons OT-2.

Automation has become increasingly vital to accommodate the growing demands of sample throughput, reliability, and reduced hands-on-time. Read More

Automated NGS Quantification, Normalization, and Pooling Using the OT-2

The Opentrons® OT-2 effectively performs qPCR quantification of NGS libraries, maintaining high precision while reducing hands-on time. Two methods of sample normalization were tested: individually for each sample or pooled with enzymatic normalization. Read More

Volatile Liquid Handling with Opentrons OT-2

Volatile liquids, liquids that evaporate readily at room temperature, are common reagents in various applications. Read More

Viscous Liquid Handling Automation using Opentrons OT-2

Molecular assays require precise pipetting reagents and samples with a diverse set of properties — including viscous ones that can clog tips, distribute unevenly and complicate reproducibility… Read More

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